Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5,8,11,14,17-eicosapentaenoic acid | competitive inhibition of oxygenation of arachidonic acid | Cavia porcellus | |
adenosine | slight stimulation | Cavia porcellus | |
ADP | stimulation | Cavia porcellus | |
AMP | stimulation | Cavia porcellus | |
ATP | stimulates | Cavia porcellus | |
cAMP | stimulation | Cavia porcellus | |
GTP | stimulation | Cavia porcellus | |
UTP | stimulation | Cavia porcellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,8,11,14,17-eicosapentaenoic acid | - |
Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
5,8,11,14,17-eicosapentaenoic acid | - |
Cavia porcellus | |
0.019 | - |
arachidonic acid | - |
Cavia porcellus | |
0.09 | - |
8,11,14-eicosatrienoic acid | - |
Cavia porcellus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Cavia porcellus | |
Ca2+ | half-maximal activity at 0.02 mM | Cavia porcellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leukocyte | peritoneal polymorphonuclear | Cavia porcellus | - |
Storage Stability | Organism |
---|---|
-70°C, 1 month, 50% loss of activity | Cavia porcellus |
0°C, 30 h, about 70% loss of activity, in presence of 2 mM ATP only 30% loss of activity | Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,8,11,14,17-eicosapentaenoic acid + O2 | - |
Cavia porcellus | ? | - |
? | |
8,11,14-eicosatrienoic acid + O2 | - |
Cavia porcellus | ? | - |
? | |
arachidonate + O2 | i.e. 5,8,11,14-eicosatetraenoic acid | Cavia porcellus | (6E,8Z,11Z,14Z)-(5S)-hydroperoxyicosa-6,8,11,14-tetraenoate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
5 min, stable up to | Cavia porcellus |
60 | - |
5 min, most of the activity is lost | Cavia porcellus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
5,8,11,14,17-eicosapentaenoic acid | - |
Cavia porcellus |