Cloned (Comment) | Organism |
---|---|
pcDNA3.1(1) vector carrying native or mutant 15-LOX-1 expressed in HCT-116 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H361L | is devoid of enzymatic activity, can induce phosphorylation of p53 as the wild-type | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Homo sapiens | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
15-lipoxygenase-1 | - |
Homo sapiens |
15-LOX-1 | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | interleukin-4 increases the expression of 15-LOX-1 and of downstream targets of p53 | up |
General Information | Comment | Organism |
---|---|---|
physiological function | 15-LOX-1 induces phosphorylation of tumor suppressor p53 independent of enzymatic activity. HCT-116 cells transiently transfected with either native or mutant 15-LOX-1 show an increase in p53 phosphorylation and an increase in the expression of downstream genes. 15-LOX-1 interacts with, and binds to, DNA-dependent protein kinase, which causes an approximate 3fold enhancement in kinase activity, resulting in increased p53 phosphorylation at Ser15 | Homo sapiens |