Activating Compound | Comment | Organism | Structure |
---|---|---|---|
12(S)-hydroperoxyeicosatetraenoic acid | reduces the kinetic lag phase | Homo sapiens | |
13(S)-hydroperoxyoctadecadienoic acid | activates 15-hLO-1 and removes the kinetic lag phase | Homo sapiens | |
15(S)-hydroperoxyeicosatetraenoic acid | activates 15-hLO-1 and removes the kinetic lag phase | Homo sapiens | |
additional information | under cellular conditions (low fatty acid and low oxygen concentrations), the allosteric binding of 12(S)-hydroxyeicosatetraenoic acid to 15-hLO-1 can increase the substrate specificity of 15-hLO-1 toward arachidonic acid over linoleic acid significantly, tunneling mechanism | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | under cellular conditions (low fatty acid and low oxygen concentrations), the allosteric binding of 12(S)-hydroxyeicosatetraenoic acid to 15-hLO-1 can increase the substrate specificity of 15-hLO-1 toward arachidonic acid over linoleic acid significantlysignificantly, which may have important implications in cancer progression | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
overexpressed as N-terminally, His6-tagged protein | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arachidonic acid | autoinactivates 15-hLO-1 to a much greater extent than linoleic acid at high substrate concentrations. No autoinactivation at low substrate concentrations | Homo sapiens | |
linoleic acid | autoinactivates 15-hLO-1 to a much greater extent than linoleic acid at high substrate concentrations. No autoinactivation at low substrate concentrations | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0096 | - |
O2 | with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C | Homo sapiens | |
0.015 | - |
O2 | with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid | Homo sapiens | |
0.022 | - |
O2 | with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid | Homo sapiens | |
0.024 | - |
O2 | with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | iron-containing metalloenzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to greater than 90% purity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
reticulocyte | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | 5fold higher affinity for arachidonic acid than for linoleic acid for 15-hLO-1 | Homo sapiens | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | - |
? | |
linoleic acid + O2 | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
15-hLO-1 | - |
Homo sapiens |
15-lipoxygenase-1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.1 | - |
O2 | with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid | Homo sapiens | |
5.6 | - |
O2 | with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C | Homo sapiens | |
7 | - |
O2 | with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid | Homo sapiens | |
7.6 | - |
O2 | with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
240 | - |
O2 | with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C | Homo sapiens | |
320 | - |
O2 | with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid | Homo sapiens | |
340 | - |
O2 | with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid | Homo sapiens | |
790 | - |
O2 | with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C | Homo sapiens |