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Literature summary for 1.13.11.33 extracted from

  • Wecksler, A.T.; Jacquot, C.; van der Donk, W.A.; Holman, T.R.
    Mechanistic investigations of human reticulocyte 15- and platelet 12-lipoxygenases with arachidonic acid (2009), Biochemistry, 48, 6259-6267.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
12(S)-hydroperoxyeicosatetraenoic acid reduces the kinetic lag phase Homo sapiens
13(S)-hydroperoxyoctadecadienoic acid activates 15-hLO-1 and removes the kinetic lag phase Homo sapiens
15(S)-hydroperoxyeicosatetraenoic acid activates 15-hLO-1 and removes the kinetic lag phase Homo sapiens
additional information under cellular conditions (low fatty acid and low oxygen concentrations), the allosteric binding of 12(S)-hydroxyeicosatetraenoic acid to 15-hLO-1 can increase the substrate specificity of 15-hLO-1 toward arachidonic acid over linoleic acid significantly, tunneling mechanism Homo sapiens

Application

Application Comment Organism
medicine under cellular conditions (low fatty acid and low oxygen concentrations), the allosteric binding of 12(S)-hydroxyeicosatetraenoic acid to 15-hLO-1 can increase the substrate specificity of 15-hLO-1 toward arachidonic acid over linoleic acid significantlysignificantly, which may have important implications in cancer progression Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed as N-terminally, His6-tagged protein Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
arachidonic acid autoinactivates 15-hLO-1 to a much greater extent than linoleic acid at high substrate concentrations. No autoinactivation at low substrate concentrations Homo sapiens
linoleic acid autoinactivates 15-hLO-1 to a much greater extent than linoleic acid at high substrate concentrations. No autoinactivation at low substrate concentrations Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0096
-
O2 with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C Homo sapiens
0.015
-
O2 with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid Homo sapiens
0.022
-
O2 with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid Homo sapiens
0.024
-
O2 with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ iron-containing metalloenzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to greater than 90% purity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
reticulocyte
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + O2 5fold higher affinity for arachidonic acid than for linoleic acid for 15-hLO-1 Homo sapiens (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
?
linoleic acid + O2
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
15-hLO-1
-
Homo sapiens
15-lipoxygenase-1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1
-
O2 with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid Homo sapiens
5.6
-
O2 with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C Homo sapiens
7
-
O2 with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid Homo sapiens
7.6
-
O2 with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
240
-
O2 with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C Homo sapiens
320
-
O2 with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid Homo sapiens
340
-
O2 with arachidonic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C, in the presence of 12(S)-hydroperoxyeicosatetraenoic acid Homo sapiens
790
-
O2 with linoleic acid as substrate, in 25 mM Hepes buffer, pH 7.5, 25°C Homo sapiens