Cloned (Comment) | Organism |
---|---|
expression of isozyme 15-hLO-1 as His-tagged enzyme, and of untagged isozyme 15-hLO-2 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics of isozymes 15-hLO-1 and 15-hLO-2 with arachidonate and linoleic acid in presence or absence of 0.2% sodium cholate, or of oxygenated fatty acids, overview | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required, iron content of the 15-hLO isozymes determined with a Finnigan inductively coupled plasma mass spectrometer, ICP-MS, using cobalt-EDTA as an internal standard | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | Homo sapiens | - |
(5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate | - |
? | |
linoleic acid + O2 | Homo sapiens | - |
(9Z,11E)-(13S)-13-hydroperoxy-octadeca-9,11-dienoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozymes 15-hLO-1 and 15-hLO-2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozyme 15-hLO-1 by nickel affinity chromatography, recombinant untagged isozyme 15-hLO-2 by ion exchange chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epithelial cell | isozyme 15-hLO-2 | Homo sapiens | - |
reticulocyte | isozyme 15-hLO-1 | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Homo sapiens | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate | - |
? | |
arachidonate + O2 | preferred substrate of isozyme 15-hLO-2, reaction of EC 1.13.11.33 | Homo sapiens | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate | - |
? | |
linoleic acid + O2 | - |
Homo sapiens | (9Z,11E)-(13S)-13-hydroperoxy-octadeca-9,11-dienoate | - |
? | |
linoleic acid + O2 | preferred substrate of isozyme 15-hLO-1, reaction of EC 1.13.11.12 | Homo sapiens | (9Z,11E)-(13S)-13-hydroperoxy-octadeca-9,11-dienoate | - |
? | |
additional information | substrate specificity of 15-hLO isozymes, the specific activity is affected by cholate and lipoxygenation reaction products, e.g. 13-hydroperoxyoctadienoic acid changes the (kcat/Km)AA/(kcat/Km)LA ratio more than 5fold for 15-hLO-1 and 3fold for 15-hLO-2, while 12-(S)-hydroperoxyeicosatetraenoic acid affects only the ratio of 15-hLO-1 more than 5fold. In addition, 13-(S)-hydroxyoctadecadienoic acid and 12-(S)-hydroxyeicosatetraenoic acid also affect substrate specificity, indicating that iron oxidation is not responsible for the change in the (kcat/Km)AA/(kcat/Km)LA ratio, residues R402, F414, F352, I417, and M418 are located at the active site and involved in catalysis, presence of a product-activated, allosteric regulatory site for both 15-hLO isozymes, competitive substrate capture experiments, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
15-hLO | - |
Homo sapiens |
15-lipoxygenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |