BRENDA - Enzyme Database show
show all sequences of 1.13.11.32

Nitronate monooxygenase, a model for anionic flavin semiquinone intermediates in oxidative catalysis

Gadda, G.; Francis, K.; Arch. Biochem. Biophys. 493, 53-61 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Cyberlindnera mrakii
expression in Escherichia coli
Neurospora crassa
Crystallization (Commentary)
Crystallization
Organism
-
Pseudomonas aeruginosa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
-
-
-
Neurospora crassa
Q01284
-
-
Cyberlindnera mrakii
Q12723
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ethyl nitronate + O2
in the oxidative pathway, the transfer of a single electron oxidizes the organic substrate and reduces the enzyme-bound flavin to an anionic semiquinone species
695891
Neurospora crassa
ethanal + nitrite
-
-
-
?
additional information
both the neutral and anionic forms of nitroalkanes act as substrates for the oxidative denitrification reaction. The enzyme is a monooxygenase
695891
Neurospora crassa
?
-
-
-
-
additional information
only alkyl nitronates are used as substrates in the oxidative denitrification reaction. Nitroalkanes are not substrates. The enzyme is a monooxygenase
695891
Cyberlindnera mrakii
?
-
-
-
-
nitroethane + O2
-
695891
Neurospora crassa
ethanal + nitrite
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
FMN
non-covalently bound flavin cofactor, the enzyme contains one mole of FMN per mole of subunit
Cyberlindnera mrakii
FMN
non-covalently bound flavin cofactor, the enzyme contains one mol of FMN per mol of subunit, the enzyme utilizes a histidine in the active site to abstract the alpha-proton of neutral substrates and to stabilize the transient anionic flavosemiquinone that forms during turnover
Neurospora crassa
FMN
the enzyme contains one mol of FMN per mol of subunit
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Cyberlindnera mrakii
expression in Escherichia coli
Neurospora crassa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMN
non-covalently bound flavin cofactor, the enzyme contains one mole of FMN per mole of subunit
Cyberlindnera mrakii
FMN
non-covalently bound flavin cofactor, the enzyme contains one mol of FMN per mol of subunit, the enzyme utilizes a histidine in the active site to abstract the alpha-proton of neutral substrates and to stabilize the transient anionic flavosemiquinone that forms during turnover
Neurospora crassa
FMN
the enzyme contains one mol of FMN per mol of subunit
Pseudomonas aeruginosa
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ethyl nitronate + O2
in the oxidative pathway, the transfer of a single electron oxidizes the organic substrate and reduces the enzyme-bound flavin to an anionic semiquinone species
695891
Neurospora crassa
ethanal + nitrite
-
-
-
?
additional information
both the neutral and anionic forms of nitroalkanes act as substrates for the oxidative denitrification reaction. The enzyme is a monooxygenase
695891
Neurospora crassa
?
-
-
-
-
additional information
only alkyl nitronates are used as substrates in the oxidative denitrification reaction. Nitroalkanes are not substrates. The enzyme is a monooxygenase
695891
Cyberlindnera mrakii
?
-
-
-
-
nitroethane + O2
-
695891
Neurospora crassa
ethanal + nitrite
-
-
-
?
Other publictions for EC 1.13.11.32
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
695891
Gadda
Nitronate monooxygenase, a mod ...
Cyberlindnera mrakii, Neurospora crassa, Pseudomonas aeruginosa
Arch. Biochem. Biophys.
493
53-61
2009
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1
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4
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696605
Francis
Kinetic evidence for an anion ...
Cyberlindnera mrakii, Neurospora crassa
Bioorg. Chem.
37
167-172
2009
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16
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16
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2
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16
16
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6
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1
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