Cloned (Comment) | Organism |
---|---|
ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) | Mus musculus |
ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) | Danio rerio |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | physico-chemical state of the substrate and the complex equilibrium between fatty acid monomers, acid soaps and micelles may impact the reaction specificity of LOX-isoforms | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | - |
- |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on Ni column | Mus musculus |
on Ni column | Danio rerio |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skin | - |
Mus musculus | - |
Storage Stability | Organism |
---|---|
-80°C, 10% glycerol | Mus musculus |
-80°C, 10% glycerol | Danio rerio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Mus musculus | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate | 12-H(p)ETE is the major reaction product of 12/15-LOX independent of the pH | ? | |
arachidonate + O2 | - |
Danio rerio | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate | 15-H(p)ETE is the major reaction product independent of the pH | ? |
Synonyms | Comment | Organism |
---|---|---|
12-lipoxygenase | - |
Mus musculus |
12-LOX | - |
Danio rerio |
12/15-LOX | - |
Mus musculus |