Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.31 extracted from

  • Walther, M.; Roffeis, J.; Jansen, C.; Anton, M.; Ivanov, I.; Kuhn, H.
    Structural basis for pH-dependent alterations of reaction specificity of vertebrate lipoxygenase isoforms (2009), Biochim. Biophys. Acta, 1791, 827-835.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) Mus musculus
ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) Danio rerio

Inhibitors

Inhibitors Comment Organism Structure
additional information physico-chemical state of the substrate and the complex equilibrium between fatty acid monomers, acid soaps and micelles may impact the reaction specificity of LOX-isoforms Mus musculus

Organism

Organism UniProt Comment Textmining
Danio rerio
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
on Ni column Mus musculus
on Ni column Danio rerio

Source Tissue

Source Tissue Comment Organism Textmining
skin
-
Mus musculus
-

Storage Stability

Storage Stability Organism
-80°C, 10% glycerol Mus musculus
-80°C, 10% glycerol Danio rerio

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + O2
-
Mus musculus (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate 12-H(p)ETE is the major reaction product of 12/15-LOX independent of the pH ?
arachidonate + O2
-
Danio rerio (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate 15-H(p)ETE is the major reaction product independent of the pH ?

Synonyms

Synonyms Comment Organism
12-lipoxygenase
-
Mus musculus
12-LOX
-
Danio rerio
12/15-LOX
-
Mus musculus