Literature summary for 1.13.11.31 extracted from

Jacquot, C.; Wecksler, A.T.; McGinley, C.M.; Segraves, E.N.; Holman, T.R.; van der Donk, W.A.
Isotope sensitive branching and kinetic isotope effects in the reaction of deuterated arachidonic acids with human 12- and 15-lipoxygenases (2008), Biochemistry, 47, 7295-7303.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of isozyme 12-hLO as His-tagged enzyme	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	required for the catalytic cycle of fatty acid oxidation by lipoxygenase, overview	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
arachidonate + O2	Homo sapiens	arachidonic acid metabolization by lipoxygenases, overview	(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P18054	isozyme platelet 12-hLO	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged isozyme 12-hLO by nickel affinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
blood platelet	isozyme 12-hLO	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
arachidonate + O2	arachidonic acid metabolization by lipoxygenases, overview	Homo sapiens	(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate	-	?
arachidonate + O2	regioselective oxidation is achieved through control over the position of hydrogen atom abstraction by the geometry and size of the enzyme active site and through control by the protein over the interaction of molecular oxygen with the generated delocalized substrate radical, analysis of catalytic mechanisms of lipoxygenases using 10,10-dideuterated arachidonic acid, 13,13-dideuterated arachidonic acid, and 0,10,13,13-d4-AA, overview	Homo sapiens	(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate	i.e. 12-HPETE, 12-hLO also forms small amounts of 11-HPETE, 8-HPETE, and 15-HPETE, overview	?
additional information	product distributions of lipoxygenases under various conditions, overview	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
12-lipoxygenase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)