Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G441A | the mutant enzyme G441A converts arachidonic acid to (8S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid and (12R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid in a 1.4:1 ratio, wild-type enzyme converts arachidonate only to (12R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid | Homo sapiens |
G441S | the mutant enzyme G441A converts arachidonic acid to 8S-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid (major product) and (12R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid (minor product), wild-type enzyme converts arachidonate only to (12R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid | Homo sapiens |
G441T | inactive mutant enzyme | Homo sapiens |
G441V | inactive mutant enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonic acid + O2 | - |
Homo sapiens | (12R)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoic acid | the mutant enzyme G441A converts arachidonic acid to 8S-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid and 12R-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid in a 1.4:1 ratio | ? |
Synonyms | Comment | Organism |
---|---|---|
12R-LOX-2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
arachidonate | mutant enzyme A416G | Homo sapiens | |
14 | - |
arachidonate | wild-type enzyme | Homo sapiens |