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Literature summary for 1.13.11.3 extracted from

  • Xu, M.; Liu, D.; Sun, P.; Li, Y.; Wu, M.; Liu, W.; Maser, E.; Xiong, G.; Guo, L.
    Degradation of 2,4,6-trinitrotoluene (TNT) involvement of protocatechuate 3,4-dioxygenase (P34O) in Buttiauxella sp. S19-1 (2021), Toxics, 9, 231 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.3

Application

Application Comment Organism
environmental protection the enzyme could play a significant role in 2,4,6-trinitrotoluene (TNT) degradation Buttiauxella sp. S19-1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Buttiauxella sp. S19-1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1009
-
 3,4-dihydroxybenzoate pH 7.2, 35°C Buttiauxella sp. S19-1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,4-dihydroxybenzoate + O2 Buttiauxella sp. S19-1
-
 3-carboxy-cis,cis-muconate
-
 ?

Organism

Organism UniProt Comment Textmining
Buttiauxella sp. S19-1
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.46
-
 pH 7.2, 35°C, recombinant protein Buttiauxella sp. S19-1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxybenzoate + O2
-
 Buttiauxella sp. S19-1 3-carboxy-cis,cis-muconate
-
 ?

Synonyms

Synonyms Comment Organism
P34O
-
 Buttiauxella sp. S19-1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
 3,4-dihydroxybenzoate pH 7.2, 35°C Buttiauxella sp. S19-1

Expression

Organism Comment Expression
Buttiauxella sp. S19-1 upregulated during 2,4,6-trinitrotoluene (TNT) degradation up

General Information

General Information Comment Organism
malfunction a knockout of the BuP34O gene to generate S-M1 mutant strain causes a marked reduction in 2,4,6-trinitrotoluene (TNT) degradation efficiency compared to the S19-1 strain Buttiauxella sp. S19-1
physiological function key enzyme in the 2-ketoadipate pathway. The involvement of the enzyme in bacterial degradation of 2,4,6-trinitrotoluene (TNT) and its metabolites Buttiauxella sp. S19-1