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Literature summary for 1.13.11.3 extracted from
- Expression and purification of nuclease-free oxygen scavenger protocatechuate 3,4-dioxygenase (2019), J. Vis. Exp., 153, 10.3791 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxybenzoate + O2 | Pseudomonas putida | - |
3-carboxy-cis,cis-muconate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| protocol for the expression and purification of highly active Pseudomonas putida protocatechuate-3,4-dioxygenase with no detectable nuclease contamination. Purification by nickel affinity chromatography, elution in 2 steps of imidazole concentrations, chromatography fractions are analyzed by SDS-PAGE, fractions of nearly pure enzyme are concentrated and further purified by size exclusion chromatography | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxybenzoate + O2 | - |
Pseudomonas putida | 3-carboxy-cis,cis-muconate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | heterodimer of hexahistidine tagged pcaH and pcaG | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PCD | - |
Pseudomonas putida |
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Release 2023.1 (January 2023)
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