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Literature summary for 1.13.11.3 extracted from
- Four aromatic intradiol ring cleavage dioxygenases from Aspergillus niger (2019), Appl. Environ. Microbiol., 85, e01786-19 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 36.3 | - |
3,4-dihydroxybenzoate | pH 7.4, 22°C | Aspergillus niger |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | Fe(III)-containing enzyme, 0.8 mol of iron per subunit | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63230 | - |
analytical ultracentrifugation | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxybenzoate + O2 | Aspergillus niger | - |
3-carboxy-cis,cis-muconate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.7 | - |
pH 7.4, 22°C | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxybenzoate + O2 | - |
Aspergillus niger | 3-carboxy-cis,cis-muconate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 35000, SDS-PAGE | Aspergillus niger |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.9 | - |
3,4-dihydroxybenzoate | pH 7.4, 22°C | Aspergillus niger | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | phylogenetically distantly related to heterodimeric bacterial protocatechuate 3,4-dioxygenases | Aspergillus niger |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
3,4-dihydroxybenzoate | pH 7.4, 22°C | Aspergillus niger | |
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Release 2023.1 (January 2023)
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