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Literature summary for 1.13.11.3 extracted from
- Highly efficient and stable novel nanobiohybrid catalyst to avert 3,4-dihydroxybenzoic acid pollutant in water (2016), Sci. Rep., 6, 33572 .
General Stability
| General Stability | Organism |
|---|---|
| multi-walled carbon nanotube-immobilized enzyme exhibits improved thermal and pH stabilities compared to the free enzyme | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.037 | - |
3,4-dihydroxybenzoate | free enzyme, at pH 9.0 and 55°C | Pseudomonas putida |  |
| 0.095 | - |
3,4-dihydroxybenzoate | multi-walled carbon nanotube-immobilized enzyme, at pH 10.0 and 60°C | Pseudomonas putida | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxybenzoate + O2 | Pseudomonas putida | - |
3-carboxy-cis,cis-muconate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 25°C, free enzyme, 30 days, 61% loss of activity | Pseudomonas putida |
| 25°C, immobilized enzyme, 30 days, 56% loss of activity | Pseudomonas putida |
| 4°C, free enzyme, 30 days, 59% loss of activity | Pseudomonas putida |
| 4°C, immobilized enzyme, 30 days, 56% loss of activity | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxybenzoate + O2 | - |
Pseudomonas putida | 3-carboxy-cis,cis-muconate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3,4-POD | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
the free enzyme loses 82% of relative activity after 180 min of incubations at 90°C | Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
3,4-dihydroxybenzoate | free enzyme, at pH 9.0 and 55°C | Pseudomonas putida | |
| 4.3 | - |
3,4-dihydroxybenzoate | multi-walled carbon nanotube-immobilized enzyme, at pH 10.0 and 60°C | Pseudomonas putida | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Pseudomonas putida |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 45.1 | - |
3,4-dihydroxybenzoate | multi-walled carbon nanotube-immobilized enzyme, at pH 10.0 and 60°C | Pseudomonas putida | |
| 93.5 | - |
3,4-dihydroxybenzoate | free enzyme, at pH 9.0 and 55°C | Pseudomonas putida | |
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