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Literature summary for 1.13.11.3 extracted from
- Protocatechuate 3,4-dioxygenase from thermophilic Geobacillus sp. strain (2007), Biologija (Vilnius), 53, 31-34.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| environmental protection | the purified enzyme can be used in bioremediation of polluted groundwater or soil contaminated with various aromatic compounds ranging from monocyclic to polycyclic | Geobacillus sp. |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
3,4-dihydroxybenzoate | - |
Geobacillus sp. |  |
| 0.033 | - |
catechol | - |
Geobacillus sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 480000 | - |
gel filtration | Geobacillus sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus sp. | - |
strain AY946034 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture-condition:naphthalene-grown cell | - |
Geobacillus sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 34.2 | - |
- |
Geobacillus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxybenzoate + O2 | - |
Geobacillus sp. | 3-carboxy-cis,cis-muconate | - |
? | |
| catechol + O2 | - |
Geobacillus sp. | muconate | - |
? | |
| additional information | no activity with gentisate | Geobacillus sp. | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Geobacillus sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 70 | 50°C: about 55% of maximal activity, 70°C: about 60% of maximal activity | Geobacillus sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life is 40 min | Geobacillus sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Geobacillus sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | pH 6.0: about 40% of maximal activity, pH 9.0: about 65% of maximal activity | Geobacillus sp. |
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Release 2023.1 (January 2023)
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