Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
American Type Culture Collection (ATCC) 23975, previously classified as Pseudomonas aeruginosa | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate | the computational (hybrid density functional method B3LYP) results of the catalytic cycle of the intradiol dioxygenases suggest: (1)binging of the substrate as a dianion (2) binding of dioxygen to the metal aided by an electron transfer from the substrate to O2 (3) formation of a bridging peroxo intermediate and its conformational change, which opens the coordination site trans to His462, (4) binding of a neutral XOH ligand (H2O or Tyr447) at the open site, (5) proton transfer from XOH to the neighboring peroxo ligand yielding the hydroperoxo intermediate, (6) a Criegee rearrangment leading to the anhydride intermediate (the criegee mechanism requires an in-plane oriantation of the involved two oxygen and two carbon atoms. Under some conditions homolytic O-O bond cleavage might compete with the Criegee rearangment.) and (7) hydrolysis of the anhydride to the final acyclic product. | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
3,4-PCD | - |
Pseudomonas putida |