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Literature summary for 1.13.11.3 extracted from

  • Hammer, A.; Stolz, A.; Knackmuss, H.J.
    Purification and characterization of a novel type of protocatechuate 3,4-dioxygenase with the ability to oxidize 4-sulfocatechol (1996), Arch. Microbiol., 166, 92-100.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-sulfocatechol competitive vs. protocatechuate Agrobacterium tumefaciens
4-sulfocatechol competitive vs. protocatechuate Hydrogenophaga palleronii
protocatechuate competitive vs. 4-sulfocatechol Agrobacterium tumefaciens
protocatechuate competitive vs. 4-sulfocatechol Hydrogenophaga palleronii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
4-sulfocatechol protocatechuate 3,4-dioxygenase type II Hydrogenophaga palleronii
0.054
-
protocatechuate protocatechuate 3,4-dioxygenase type I Agrobacterium tumefaciens
0.058
-
protocatechuate protocatechuate 3,4-dioxygenase type II Hydrogenophaga palleronii
0.058
-
4-sulfocatechol protocatechuate 3,4-dioxygenase type II Agrobacterium tumefaciens
0.205
-
protocatechuate protocatechuate 3,4-dioxygenase type II Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
protocatechuate 3,4-dioxygenase I and II
-
Agrobacterium tumefaciens S2
-
protocatechuate 3,4-dioxygenase I and II
-
Hydrogenophaga palleronii
-
protocatechuate 3,4-dioxygenase II
-
Hydrogenophaga palleronii S1
-
protocatechuate 3,4-dioxygenase II
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose FF, ammonium sulfate, Sephacryl S-300HR, phenyl-superose HR5/5, Mono Q, type II protocatechuate 3,4-dioxygenase Agrobacterium tumefaciens
Q-Sepharose FF, ammonium sulfate, Sephacryl S-300HR, Phenyl-Superose, type I protocatechuate 3,4-dioxygenase Agrobacterium tumefaciens
Q-Sepharose FF, ammonium sulfate, Superdex 200, Fractogel, type II protocatechuate 3,4-dioxygenase Hydrogenophaga palleronii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.2
-
type II protocatechuate 3,4-dioxygenase, oxidation of 4-sulfocatechol Hydrogenophaga palleronii
16.7
-
type II protocatechuate 3,4-dioxygenase, oxidation of 4-sulfocatechol Agrobacterium tumefaciens
105
-
type I protocatechuate 3,4-dioxygenase Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxybenzoate + O2
-
Agrobacterium tumefaciens 3-carboxy-cis,cis-muconate
-
?
3,4-dihydroxybenzoate + O2
-
Hydrogenophaga palleronii 3-carboxy-cis,cis-muconate
-
?
3,4-dihydroxybenzoate + O2
-
Agrobacterium tumefaciens S2 3-carboxy-cis,cis-muconate
-
?
3,4-dihydroxybenzoate + O2
-
Hydrogenophaga palleronii S1 3-carboxy-cis,cis-muconate
-
?
3,4-dihydroxymandelic acid + O2 protocatechuate 3,4-dioxygenase II Hydrogenophaga palleronii ?
-
?
3,4-dihydroxymandelic acid + O2 protocatechuate 3,4-dioxygenase II Hydrogenophaga palleronii S1 ?
-
?
3,4-dihydroxyphenylacetate + O2
-
Agrobacterium tumefaciens ?
-
?
3,4-dihydroxyphenylacetate + O2
-
Hydrogenophaga palleronii ?
-
?
3-(3,4-dihydroxyphenyl)propionate + O2 protocatechuate 3,4-dioxygenase II Hydrogenophaga palleronii ?
-
?
3-methylcatechol + O2
-
Agrobacterium tumefaciens 2-methylmuconate
-
?
3-methylcatechol + O2
-
Hydrogenophaga palleronii 2-methylmuconate
-
?
3-methylcatechol + O2
-
Agrobacterium tumefaciens S2 2-methylmuconate
-
?
3-methylcatechol + O2
-
Hydrogenophaga palleronii S1 2-methylmuconate
-
?
4-methylcatechol + O2 at very low rates Agrobacterium tumefaciens 3-methyl-cis,cis-muconate
-
?
4-methylcatechol + O2 at very low rates Hydrogenophaga palleronii 3-methyl-cis,cis-muconate
-
?
4-methylcatechol + O2 at very low rates Agrobacterium tumefaciens S2 3-methyl-cis,cis-muconate
-
?
4-methylcatechol + O2 at very low rates Hydrogenophaga palleronii S1 3-methyl-cis,cis-muconate
-
?
4-sulfocatechol + O2 catalyzed by protocatechuate 3,4-dioxygenase type II only Agrobacterium tumefaciens 3-sulfomuconate
-
?
4-sulfocatechol + O2 catalyzed by protocatechuate 3,4-dioxygenase type II only Hydrogenophaga palleronii 3-sulfomuconate
-
?
4-sulfocatechol + O2 catalyzed by protocatechuate 3,4-dioxygenase type II only Agrobacterium tumefaciens S2 3-sulfomuconate
-
?
catechol + O2
-
Agrobacterium tumefaciens muconate
-
?
catechol + O2
-
Hydrogenophaga palleronii muconate
-
?
catechol + O2
-
Agrobacterium tumefaciens S2 muconate
-
?
catechol + O2
-
Hydrogenophaga palleronii S1 muconate
-
?
trans-3,4-dihydroxycinnamate + O2
-
Agrobacterium tumefaciens ?
-
?
trans-3,4-dihydroxycinnamate + O2
-
Hydrogenophaga palleronii ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.6
-
4-sulfocatechol protocatechuate 3,4-dioxygenase type II Agrobacterium tumefaciens
18.8
-
protocatechuate protocatechuate 3,4-dioxygenase type II Agrobacterium tumefaciens
89.5
-
protocatechuate protocatechuate 3,4-dioxygenase type I Agrobacterium tumefaciens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.032
-
4-sulfocatechol protocatechuate 3,4-dioxygenase type II Hydrogenophaga palleronii
0.053
-
4-sulfocatechol protocatechuate 3,4-dioxygenase type II Agrobacterium tumefaciens
0.066
-
protocatechuate protocatechuate 3,4-dioxygenase type II Hydrogenophaga palleronii
0.102
-
protocatechuate protocatechuate 3,4-dioxygenase type II Agrobacterium tumefaciens