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Literature summary for 1.13.11.3 extracted from
- Brevibacterium fuscum protocatechuate 3,4-dioxygenase. Purification, crystallization, and characterization (1984), J. Biol. Chem., 259, 4466-4475.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| microdialysis of approx. 0.05 ml of enzyme solution, 80 mg/ml against 10 ml volumes of buffered solutions, large single crystals | Brevibacterium fuscum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.125 | - |
protocatechuate | - |
Brevibacterium fuscum |  |
| 0.8 | - |
O2 | - |
Brevibacterium fuscum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | 5 Fe atoms per holoenzyme | Brevibacterium fuscum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22500 | - |
alpha,beta, 5 * 22500 + 5 * 40000, SDS-PAGE | Brevibacterium fuscum |
| 40000 | - |
alpha,beta, 5 * 22500 + 5 * 40000, SDS-PAGE | Brevibacterium fuscum |
| 315000 | - |
analytical ultracentrifugation | Brevibacterium fuscum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevibacterium fuscum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | carbohydrate contributes less than 0.2% to the mass of the holoenzyme | Brevibacterium fuscum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Brevibacterium fuscum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 33 | - |
- |
Brevibacterium fuscum |
| 41 | - |
- |
Brevibacterium fuscum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxybenzoate + O2 | - |
Brevibacterium fuscum | 3-carboxy-cis,cis-muconate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| decamer | alpha,beta, 5 * 22500 + 5 * 40000, SDS-PAGE | Brevibacterium fuscum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 417 | - |
3,4-dihydroxybenzoate | - |
Brevibacterium fuscum | |
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