Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sphingobium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DEPC | 1 mM, no residual activity | Sphingobium sp. | |
EDTA | 1 mM, no residual activity | Sphingobium sp. | |
isoxaflutole | - |
Sphingobium sp. | |
mesotrione | - |
Sphingobium sp. | |
additional information | not inhibitory at 1 mM: urea, PMSF | Sphingobium sp. | |
tembotrione | - |
Sphingobium sp. | |
topramezone | - |
Sphingobium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
4-hydroxyphenylpyruvate | pH 6, 35°C | Sphingobium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 66.5% of the activity with Fe2+ | Sphingobium sp. | |
Fe2+ | required | Sphingobium sp. | |
Fe3+ | 95% of the reactivity with Fe2+ | Sphingobium sp. | |
Mg2+ | 101% of the activity with Fe2+ | Sphingobium sp. | |
Mn2+ | 22% of the activity with Fe2+ | Sphingobium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Sphingobium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium sp. | A0A6B9L0W0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, using Co2+-affinity chromatography | Sphingobium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | - |
Sphingobium sp. | homogentisate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE, 1 * 38800, calculated from sequence | Sphingobium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
4-hydroxyphenylpyruvate | pH 6, 35°C | Sphingobium sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
tembotrione | pH 6, 35°C | Sphingobium sp. | |
0.0013 | - |
mesotrione | pH 6, 35°C | Sphingobium sp. | |
0.0025 | - |
topramezone | pH 6, 35°C | Sphingobium sp. | |
0.006 | - |
isoxaflutole | pH 6, 35°C | Sphingobium sp. |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0025 | - |
pH 6, 35°C | Sphingobium sp. | tembotrione | |
0.0042 | - |
pH 6, 35°C | Sphingobium sp. | mesotrione | |
0.0052 | - |
pH 6, 35°C | Sphingobium sp. | topramezone | |
0.0087 | - |
pH 6, 35°C | Sphingobium sp. | isoxaflutole |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
181.2 | - |
4-hydroxyphenylpyruvate | pH 6, 35°C | Sphingobium sp. |