Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteamine | - |
Rattus norvegicus | |
diethyldithiocarbamate | without protein-A: 30% activation at 0.1 mM | Rattus norvegicus | |
DL-homocysteine | - |
Rattus norvegicus | |
L-cysteine | activates the purified enzyme under anaerobic conditions | Rattus norvegicus | |
N-acetyl-L-cysteine | - |
Rattus norvegicus | |
Neocuproine | without protein-A: slight activation at 0.1 mM | Rattus norvegicus | |
S-methyl-L-cysteine | - |
Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | with protein-A: 100% inhibition at 0.1 mM, without protein-A: 58% inhibition at 0.1 mM | Rattus norvegicus | |
8-hydroxyquinoline | with protein-A: 100% inhibition at 0.1 mM, without protein-A: 99% inhibition at 0.1 mM | Rattus norvegicus | |
Bathocuproine sulfonate | with protein-A: 5% inhibition at 0.1 mM, without protein-A: 38% inhibition at 0.1 mM | Rattus norvegicus | |
bathophenanthroline sulfonate | with protein-A: 88% inhibition, without protein-A: 100% inhibition | Rattus norvegicus | |
Carboxyethyl-L-cysteine | 53% inhibition at 1 mM | Rattus norvegicus | |
carboxymethyl-L-cysteine | 37% inhibition at 1 mM | Rattus norvegicus | |
cysteamine | 39% inhibition at 10 mM | Rattus norvegicus | |
D-cysteine | 47% inhibition at 1 mM | Rattus norvegicus | |
diethyldithiocarbamate | with protein-A: not inhibitory at 0.1 mM | Rattus norvegicus | |
DL-homocysteine | 87% inhibition at 10 mM | Rattus norvegicus | |
EDTA | with protein-A: 97% inhibition at 0.1 mM, with protein-A: 99% inhibition at 0.1 mM | Rattus norvegicus | |
EGTA | with protein-A: 100% inhibition at 0.1 mM, without protein-A: 95% inhibition at 0.1 mM | Rattus norvegicus | |
N-acetyl-L-cysteine | 35% inhibition at 10 mM | Rattus norvegicus | |
Neocuproine | with protein-A: 18% inhibition at 0.1 mM, without protein-A: slight activation at 0.1 mM | Rattus norvegicus | |
o-phenanthroline | without protein-A: 96% inhibition at 0.1 mM, with protein A: 100% inhibition at 0.1 mM | Rattus norvegicus | |
S-methyl-L-cysteine | 34% inhibition at 1 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
cysteine | in the presence of protein-A | Rattus norvegicus | |
0.67 | - |
cysteine | in the absence of protein-A | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | contains 1 atom of Fe per mol of enzyme | Rattus norvegicus | |
Fe | Fe2+ required, 0.8-0.9 gatom of iron per 22500 g of purified protein | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22500 | - |
1 * 22500, SDS-PAGE | Rattus norvegicus |
23000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
rapid and irreversible inactivation under aerobic conditions, inactivation can be prevented by a distinct cytoplasmic protein, i.e. protein A | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
using acetone precipitation, first chromatography on DEAE-cellulose column, second chromatography on DEAE-cellulose column, chromatography on Sephadex G-100 column, hydroxyapatite column, DEAE-Sephadex A-25 column and Sephadex G-75 column | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
0°C, no significant loss of activity after 1 month | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + O2 | - |
Rattus norvegicus | 3-sulfino-L-alanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 22500, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
for the anaerobic activation of purified enzyme by L-cysteine | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | protein-A added to the assay mixture | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
D-cysteine | - |
Rattus norvegicus | |
0.75 | - |
S-methyl-L-cysteine | - |
Rattus norvegicus | |
1.5 | - |
cysteamine | - |
Rattus norvegicus |