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Literature summary for 1.13.11.2 extracted from

  • Adewale, P.; Lang, A.; Huang, F.; Zhu, D.; Sun, J.; Ngadi, M.; Yang, T.C.
    A novel Bacillus ligniniphilus catechol 2,3-dioxygenase shows unique substrate preference and metal requirement (2021), Sci. Rep., 11, 23982 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Halalkalibacterium ligniniphilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.193
-
 3-ethylcatechol pH 7.4, 32.5°C Halalkalibacterium ligniniphilum
0.418
-
 3-methylcatechol pH 7.4, 32.5°C Halalkalibacterium ligniniphilum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ at 1 mM, Fe2+ leads to 22fold activity increase Halalkalibacterium ligniniphilum
Mn2+ at 1 mM, Mn2+ leads to 230fold activity increase Halalkalibacterium ligniniphilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31825
-
 calculated from sequence, wild-type enzyme Halalkalibacterium ligniniphilum
32000
-
 gel filtration Halalkalibacterium ligniniphilum
33988
-
 calculated from sequence, recombinant His-tagged enzyme Halalkalibacterium ligniniphilum
34000
-
 SDS-PAGE, recombinant His-tagged enzyme Halalkalibacterium ligniniphilum

Organism

Organism UniProt Comment Textmining
Halalkalibacterium ligniniphilum WP_017726464.1
-
-
Halalkalibacterium ligniniphilum L1 WP_017726464.1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Halalkalibacterium ligniniphilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0082
-
 substrate: catechol, pH 7.4, 32.5°C Halalkalibacterium ligniniphilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxybiphenylcatechol + O2 % of the activity as compared to 3-ethylcatechol Halalkalibacterium ligniniphilum ?
-
 ?
3-ethylcatechol + O2 best substrate. The enzyme can cleave the catechol ring when substitutions occur at C3 more efficiently than C4 substitutes. The enzyme can cleave aromatic compounds with two 6-carbon ring structures Halalkalibacterium ligniniphilum ?
-
 ?
3-isopropylcatechol + O2 50% of the activity as compared to 3-ethylcatechol Halalkalibacterium ligniniphilum ?
-
 ?
3-isopropylcatechol + O2 50% of the activity as compared to 3-ethylcatechol Halalkalibacterium ligniniphilum L1 ?
-
 ?
3-methylcatechol + O2 62.5% of the activity as compared to 3-ethylcatechol Halalkalibacterium ligniniphilum 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
3-methylcatechol + O2 62.5% of the activity as compared to 3-ethylcatechol Halalkalibacterium ligniniphilum L1 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
4-methylcatechol + O2 2.7% of the activity as compared to 3-ethylcatechol Halalkalibacterium ligniniphilum 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate
-
 ?
4-methylcatechol + O2 2.7% of the activity as compared to 3-ethylcatechol Halalkalibacterium ligniniphilum L1 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate
-
 ?
catechol + O2 1.5% of the activity as compared to 3-ethylcatechol Halalkalibacterium ligniniphilum 2-hydroxymuconate-6-semialdehyde
-
 ?
catechol + O2 1.5% of the activity as compared to 3-ethylcatechol Halalkalibacterium ligniniphilum L1 2-hydroxymuconate-6-semialdehyde
-
 ?
additional information no activities observed for pyrogallol and 3-fluorocatechol Halalkalibacterium ligniniphilum ?
-
-
additional information no activities observed for pyrogallol and 3-fluorocatechol Halalkalibacterium ligniniphilum L1 ?
-
-

Subunits

Subunits Comment Organism
monomer under native conditions Halalkalibacterium ligniniphilum

Synonyms

Synonyms Comment Organism
C23Os
-
 Halalkalibacterium ligniniphilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32.5
-
-
 Halalkalibacterium ligniniphilum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 25°C: about 90% of maximal activity, 45°C: about 60% of maximal activity Halalkalibacterium ligniniphilum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
32.5 35 3 h, no loss of activity Halalkalibacterium ligniniphilum
40
-
 half-life: 231 min Halalkalibacterium ligniniphilum
52
-
 half-life: 116 min Halalkalibacterium ligniniphilum
54
-
 half-life: 32 min Halalkalibacterium ligniniphilum
56
-
 half-life: 4 min Halalkalibacterium ligniniphilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 3-methylcatechol pH 7.4, 32.5°C Halalkalibacterium ligniniphilum
0.47
-
 3-ethylcatechol pH 7.4, 32.5°C Halalkalibacterium ligniniphilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Halalkalibacterium ligniniphilum

pI Value

Organism Comment pI Value Maximum pI Value
Halalkalibacterium ligniniphilum calculated from sequence, recombinant His-tagged enzyme
-
 5.39
Halalkalibacterium ligniniphilum calculated from sequence, wild-type enzyme
-
 5.5

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.48
-
 3-methylcatechol pH 7.4, 32.5°C Halalkalibacterium ligniniphilum
2.4
-
 3-ethylcatechol pH 7.4, 32.5°C Halalkalibacterium ligniniphilum