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Literature summary for 1.13.11.2 extracted from
- Catechol 2,3-dioxygenase from a new phenolic compound degrader Thauera sp. K11 purification and biochemical characterization (2018), J. Basic Microbiol., 58, 255-262 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 5 mM, 42.2% of original activity | Thauera sp. K11 |  |
| Ca2+ | 5 mM, 93.1% of original activity | Thauera sp. K11 | |
| Fe2+ | 5 mM, 47.4% of original activity | Thauera sp. K11 | |
| Fe3+ | 5 mM, 99.4% of original activity | Thauera sp. K11 | |
| Hg2+ | 5 mM, 74.1% of original activity | Thauera sp. K11 | |
| Pb2+ | 5 mM, 85.3% of original activity | Thauera sp. K11 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02036 | - |
catechol | pH 8.0, 30°C | Thauera sp. K11 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 5 mM, 101.7% of original activity | Thauera sp. K11 | |
| Cu2+ | 5 mM, 232.8% of original activity | Thauera sp. K11 | |
| Iron | the enzyme contains 0.92 iron atoms per subunit | Thauera sp. K11 | |
| Mg2+ | 5 mM, 118.1% of original activity | Thauera sp. K11 | |
| Mn2+ | 5 mM, 195.7% of original activity | Thauera sp. K11 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 140000 | - |
gel filtration | Thauera sp. K11 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| catechol + O2 | Thauera sp. K11 | - |
2-hydroxymuconate-6-semialdehyde | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thauera sp. K11 | A0A290ZJW2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thauera sp. K11 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 27.2 | - |
pH 8.0, 30°C | Thauera sp. K11 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,5-dichlorocatechol + O2 | 30.8% of the activity as compared to catechol | Thauera sp. K11 | 2-hydroxy-3,5-dimethyl-6-oxohexa-2,4-dienoic acid | - |
? | |
| 3-chlorocatechol + O2 | 82.3% of the activity as compared to catechol | Thauera sp. K11 | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
| 3-methylcatechol + O2 | 90.8% of the activity as compared to catechol | Thauera sp. K11 | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
| 4,5-dichlorocatechol + O2 | 23.4% of the activity as compared to catechol | Thauera sp. K11 | ? | - |
? | |
| 4-chlorocatechol + O2 | 71.1% of the activity as compared to catechol | Thauera sp. K11 | 5-chloro-2-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
| 4-methylcatechol + O2 | 67.7% of the activity as compared to catechol | Thauera sp. K11 | 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate | - |
? | |
| catechol + O2 | - |
Thauera sp. K11 | 2-hydroxymuconate-6-semialdehyde | - |
? | |
| additional information | no activity with 2,3-dihydroxybiphenyl and 3,4-dihydrophenylacetate | Thauera sp. K11 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 35000, SDS-PAGE | Thauera sp. K11 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C23o | - |
Thauera sp. K11 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Thauera sp. K11 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | 20°C: about 55% of maximal activity, 60°C: about 40% of maximal activity | Thauera sp. K11 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
2 h, stable below | Thauera sp. K11 |
| 60 | - |
enzyme retains nearly 50% activity after 120 min | Thauera sp. K11 |
| 70 | - |
enzyme loses its activity within 60 min | Thauera sp. K11 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
- |
Thauera sp. K11 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | pH 5.5: about 35% of maximal activity, pH 9.5: about 35% of maximal activity | Thauera sp. K11 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 11 | 4°C, 12 h, stable | Thauera sp. K11 |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thauera sp. K11 | isoelectric focusing | 6.42 | 6.14 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | important enzyme in many bacterial pathways for the degradation of aromatic compounds | Thauera sp. K11 |
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