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Literature summary for 1.13.11.2 extracted from

  • Guo, G.; Fang, T.; Wang, C.; Huang, Y.; Tian, F.; Cui, Q.; Wang, H.
    Isolation and characterization of two novel halotolerant catechol 2,3-dioxygenases from a halophilic bacterial consortium (2015), Sci. Rep., 5, 17603 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells uncultured bacterium

General Stability

General Stability Organism
the two isoforms C23O1 and C23O2 are both highly active over a broad range of NaCl concentration, even as high as 30% (w/v) salinity, while C23O1 presents a little higher activity than C23O2 uncultured bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ the enzyme activity increases in the presence of Al3+ uncultured bacterium
Cu2+ the enzyme activity increases in the presence of Cu2+ uncultured bacterium
Fe2+ the enzyme activity increases in the presence of Fe2+ (isoform C23O1 shows 265.65% activity at 5 mM) uncultured bacterium
Fe3+ the enzyme activity increases in the presence of Fe3+ (isoform C23O2 shows 377.37% activity at 5 mM) uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2 isoform C23O1 shows 49.5% activity compared to catechol, isoform C23O2 shows 239.2% activity compared to catechol uncultured bacterium 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
4-chlorocatechol + O2 isoform C23O1 shows 50.8% activity compared to catechol, isoform C23O2 shows 226.4% activity compared to catechol uncultured bacterium 4-chloro-2-hydroxymuconate semialdehyde
-
 ?
4-methylcatechol + O2 best substrate for isoform C23O2 (280.7% activity compared to catechol), while isoform C23O1 shows 87.4% activity compared to catechol uncultured bacterium 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
catechol + O2 best substrate for isoform C23O1 (100% activity) uncultured bacterium 2-hydroxymuconate-6-semialdehyde
-
 ?
additional information the two isoforms cannot oxidize 1, 2-dihydroxynaphthalene uncultured bacterium ?
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE uncultured bacterium

Synonyms

Synonyms Comment Organism
C23O1 isoform uncultured bacterium
C23O2 isoform uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 isoform C23O1 uncultured bacterium
60
-
 isoform C23O2 uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 30 the two isoforms C23O1 and C23O2 are both highly stable in the temperature range of 4-30°C for 24 h. Their activities are kept best at 10°C and the residual activities are about 80% at 24 h, followed by 20°C with approximately 60% residual activies at 24 h. Their enzyme activities decline rapidly as the temperature increases above 50°C uncultured bacterium