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Literature summary for 1.13.11.2 extracted from

  • Hassan, H.; Zein Eldein, A.; Rizk, N.
    Cloning and kinetic properties of Catechol 2,3-dioxygenase from novel alkaliphilic BTEX-degrading Pseudomonas sp. HB01 (2014), Life Sci. J., 11, 376-384 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Pseudomonas sp. HB01

Inhibitors

Inhibitors Comment Organism Structure
3-chlorocatechol
-
Pseudomonas sp. HB01

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
2,2',3-trihydroxybiphenylether pH and temperature not specified in the publication Pseudomonas sp. HB01
0.0012
-
2,3-Dihydroxybiphenyl pH and temperature not specified in the publication Pseudomonas sp. HB01
0.03
-
3-methylcatechol pH and temperature not specified in the publication Pseudomonas sp. HB01
0.3
-
catechol pH and temperature not specified in the publication Pseudomonas sp. HB01

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
catechol + O2 Pseudomonas sp. HB01
-
2-hydroxymuconate-6-semialdehyde
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. HB01 T1T1S4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2',3-trihydroxybiphenylether + O2
-
Pseudomonas sp. HB01 ?
-
?
2,3-dihydroxybiphenyl + O2
-
Pseudomonas sp. HB01 ?
-
?
3-methylcatechol + O2
-
Pseudomonas sp. HB01 2-hydroxy-6-oxohepta-2,4-dienoate
-
?
4-chlorocatechol + O2
-
Pseudomonas sp. HB01 5-chloro-2-hydroxymuconic semialdehyde
-
?
catechol + O2
-
Pseudomonas sp. HB01 2-hydroxymuconate-6-semialdehyde
-
?

Synonyms

Synonyms Comment Organism
C2,3O
-
Pseudomonas sp. HB01
catechol 2,3 dioxygenase
-
Pseudomonas sp. HB01

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
60
-
2,2',3-trihydroxybiphenylether pH and temperature not specified in the publication Pseudomonas sp. HB01
150
-
3-methylcatechol pH and temperature not specified in the publication Pseudomonas sp. HB01
200
-
2,3-Dihydroxybiphenyl pH and temperature not specified in the publication Pseudomonas sp. HB01
360
-
catechol pH and temperature not specified in the publication Pseudomonas sp. HB01