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Literature summary for 1.13.11.2 extracted from

  • Wojcieszynska, D.; Hupert-Kocurek, K.; Jankowska, A.; Guzik, U.
    Properties of catechol 2,3-dioxygenase from crude extract of Stenotrophomonas maltophilia strain KB2 immobilized in calcium alginate hydrogels (2012), Biochem. Eng. J., 66, 1-7.
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
additional information immobilization of catechol 2,3-dioxygenase from KB2 strain using calcium alginate, the iimmobilized enzyme shows relatively higher activity against 3-methylcatechol, 4-methylcatechol, 4,5-dichlorocatechol, 3,5-dichlorocatechol, hydroquinone and tetrachlorohydroquinone than soluble enzyme, immobilization protects the enzyme from the inhibition and enhanced its resistance to inactivation during catalysis Stenotrophomonas maltophilia

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 the immobilized enzyme is less sensitive compared to the free enzyme Stenotrophomonas maltophilia
H2O2 the immobilized enzyme is less sensitive compared to the free enzyme Stenotrophomonas maltophilia
NaN3 the immobilized enzyme is less sensitive compared to the free enzyme Stenotrophomonas maltophilia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
4-methylcatechol pH 8.0, 35°C, immobilized enzyme Stenotrophomonas maltophilia
0.0425
-
4-methylcatechol pH 8.0, 35°C, free enzyme Stenotrophomonas maltophilia

Organism

Organism UniProt Comment Textmining
Stenotrophomonas maltophilia
-
-
-
Stenotrophomonas maltophilia KB2
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
355.8
-
substrate 4-methylcatechol, pH 8.0, 35°C, free enzyme Stenotrophomonas maltophilia
936.3
-
substrate 4-methylcatechol, pH 8.0, 35°C, immobilized enzyme Stenotrophomonas maltophilia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dichlorophenol + O2
-
Stenotrophomonas maltophilia ?
-
?
2,4-dichlorophenol + O2
-
Stenotrophomonas maltophilia KB2 ?
-
?
2-chlorophenol + O2
-
Stenotrophomonas maltophilia ?
-
?
2-chlorophenol + O2
-
Stenotrophomonas maltophilia KB2 ?
-
?
2-methylphenol + O2
-
Stenotrophomonas maltophilia ?
-
?
3,5-dichlorocatechol + O2 immobilized enzyme, no activity with the free enzyme Stenotrophomonas maltophilia ?
-
?
3,5-dichlorocatechol + O2 immobilized enzyme, no activity with the free enzyme Stenotrophomonas maltophilia KB2 ?
-
?
3-methylcatechol + O2
-
Stenotrophomonas maltophilia ?
-
?
3-methylcatechol + O2
-
Stenotrophomonas maltophilia KB2 ?
-
?
3-methylphenol + O2
-
Stenotrophomonas maltophilia ?
-
?
4,5-dichlorocatechol + O2
-
Stenotrophomonas maltophilia ?
-
?
4-chlorophenol + O2
-
Stenotrophomonas maltophilia ?
-
?
4-methylcatechol + O2
-
Stenotrophomonas maltophilia ?
-
?
butanol + O2
-
Stenotrophomonas maltophilia ?
-
?
catechol + O2
-
Stenotrophomonas maltophilia 2-hydroxymuconate-6-semialdehyde
-
?
ethanol + O2
-
Stenotrophomonas maltophilia ?
-
?
hydroquinone + O2 immobilized enzyme, no activity with the free enzyme Stenotrophomonas maltophilia ?
-
?
hydroquinone + O2 immobilized enzyme, no activity with the free enzyme Stenotrophomonas maltophilia KB2 ?
-
?
methanol + O2
-
Stenotrophomonas maltophilia ?
-
?
propanol + O2
-
Stenotrophomonas maltophilia ?
-
?
tetrachlorohydroquinone + O2 immobilized enzyme, no activity with the free enzyme Stenotrophomonas maltophilia ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
assay at Stenotrophomonas maltophilia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
both free and the immobilized enzyme Stenotrophomonas maltophilia

General Information

General Information Comment Organism
additional information there is a hydrophobic channel in the active site of catechol 2,3-dioxygenase. Through this channel catechol penetrates into the active site of the enzyme Stenotrophomonas maltophilia