Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization of catechol 2,3-dioxygenase from KB2 strain using calcium alginate, the iimmobilized enzyme shows relatively higher activity against 3-methylcatechol, 4-methylcatechol, 4,5-dichlorocatechol, 3,5-dichlorocatechol, hydroquinone and tetrachlorohydroquinone than soluble enzyme, immobilization protects the enzyme from the inhibition and enhanced its resistance to inactivation during catalysis | Stenotrophomonas maltophilia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | the immobilized enzyme is less sensitive compared to the free enzyme | Stenotrophomonas maltophilia | |
H2O2 | the immobilized enzyme is less sensitive compared to the free enzyme | Stenotrophomonas maltophilia | |
NaN3 | the immobilized enzyme is less sensitive compared to the free enzyme | Stenotrophomonas maltophilia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
4-methylcatechol | pH 8.0, 35°C, immobilized enzyme | Stenotrophomonas maltophilia | |
0.0425 | - |
4-methylcatechol | pH 8.0, 35°C, free enzyme | Stenotrophomonas maltophilia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Stenotrophomonas maltophilia | - |
- |
- |
Stenotrophomonas maltophilia KB2 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
355.8 | - |
substrate 4-methylcatechol, pH 8.0, 35°C, free enzyme | Stenotrophomonas maltophilia |
936.3 | - |
substrate 4-methylcatechol, pH 8.0, 35°C, immobilized enzyme | Stenotrophomonas maltophilia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dichlorophenol + O2 | - |
Stenotrophomonas maltophilia | ? | - |
? | |
2,4-dichlorophenol + O2 | - |
Stenotrophomonas maltophilia KB2 | ? | - |
? | |
2-chlorophenol + O2 | - |
Stenotrophomonas maltophilia | ? | - |
? | |
2-chlorophenol + O2 | - |
Stenotrophomonas maltophilia KB2 | ? | - |
? | |
2-methylphenol + O2 | - |
Stenotrophomonas maltophilia | ? | - |
? | |
3,5-dichlorocatechol + O2 | immobilized enzyme, no activity with the free enzyme | Stenotrophomonas maltophilia | ? | - |
? | |
3,5-dichlorocatechol + O2 | immobilized enzyme, no activity with the free enzyme | Stenotrophomonas maltophilia KB2 | ? | - |
? | |
3-methylcatechol + O2 | - |
Stenotrophomonas maltophilia | ? | - |
? | |
3-methylcatechol + O2 | - |
Stenotrophomonas maltophilia KB2 | ? | - |
? | |
3-methylphenol + O2 | - |
Stenotrophomonas maltophilia | ? | - |
? | |
4,5-dichlorocatechol + O2 | - |
Stenotrophomonas maltophilia | ? | - |
? | |
4-chlorophenol + O2 | - |
Stenotrophomonas maltophilia | ? | - |
? | |
4-methylcatechol + O2 | - |
Stenotrophomonas maltophilia | ? | - |
? | |
butanol + O2 | - |
Stenotrophomonas maltophilia | ? | - |
? | |
catechol + O2 | - |
Stenotrophomonas maltophilia | 2-hydroxymuconate-6-semialdehyde | - |
? | |
ethanol + O2 | - |
Stenotrophomonas maltophilia | ? | - |
? | |
hydroquinone + O2 | immobilized enzyme, no activity with the free enzyme | Stenotrophomonas maltophilia | ? | - |
? | |
hydroquinone + O2 | immobilized enzyme, no activity with the free enzyme | Stenotrophomonas maltophilia KB2 | ? | - |
? | |
methanol + O2 | - |
Stenotrophomonas maltophilia | ? | - |
? | |
propanol + O2 | - |
Stenotrophomonas maltophilia | ? | - |
? | |
tetrachlorohydroquinone + O2 | immobilized enzyme, no activity with the free enzyme | Stenotrophomonas maltophilia | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Stenotrophomonas maltophilia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
both free and the immobilized enzyme | Stenotrophomonas maltophilia |
General Information | Comment | Organism |
---|---|---|
additional information | there is a hydrophobic channel in the active site of catechol 2,3-dioxygenase. Through this channel catechol penetrates into the active site of the enzyme | Stenotrophomonas maltophilia |