Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains Fe2+ as a cofactor | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
4 * 39000, SDS-PAGE | Pseudomonas putida |
128000 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P06622 | - |
- |
Pseudomonas putida SH1 | P06622 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography, phenyl-Superose gel filtration, and Sephacryl S-200 gel filtration | Pseudomonas putida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:naphthalene-grown cell | - |
Pseudomonas putida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15 | - |
crude extract, in 20 mM NaH2PO4, pH 7.5, containing 1 mM catechol at 50°C | Pseudomonas putida |
802 | - |
after 52.7fold purification, in 20 mM NaH2PO4, pH 7.5, containing 1 mM catechol at 50°C | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | - |
Pseudomonas putida | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | - |
Pseudomonas putida SH1 | 2-hydroxymuconate semialdehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 39000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
C23o | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Pseudomonas putida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 80 | the enzyme activity increases starting from 25°C and reaches its maximum at 50°C, the enzyme starts to denature at 55°C and the structure is destroyed by the time the temperature reaches 80°C | Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | the half-life of the enzyme activity at 30°C is about 7 h and the half-life of the enzyme activity at 50°C is 45 min | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Pseudomonas putida |