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Literature summary for 1.13.11.2 extracted from
- Thermal effects on the activity and structural conformation of catechol 2,3-dioxygenase from Pseudomonas putida SH1 (2010), J. Phys. Chem. B, 114, 987-992.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | the enzyme contains Fe2+ as a cofactor | Pseudomonas putida |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
4 * 39000, SDS-PAGE | Pseudomonas putida |
| 128000 | - |
gel filtration | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | P06622 | - |
- |
| Pseudomonas putida SH1 | P06622 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose column chromatography, phenyl-Superose gel filtration, and Sephacryl S-200 gel filtration | Pseudomonas putida |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:naphthalene-grown cell | - |
Pseudomonas putida | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15 | - |
crude extract, in 20 mM NaH2PO4, pH 7.5, containing 1 mM catechol at 50°C | Pseudomonas putida |
| 802 | - |
after 52.7fold purification, in 20 mM NaH2PO4, pH 7.5, containing 1 mM catechol at 50°C | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| catechol + O2 | - |
Pseudomonas putida | 2-hydroxymuconate semialdehyde | - |
? | |
| catechol + O2 | - |
Pseudomonas putida SH1 | 2-hydroxymuconate semialdehyde | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 39000, SDS-PAGE | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C23o | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Pseudomonas putida |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 80 | the enzyme activity increases starting from 25°C and reaches its maximum at 50°C, the enzyme starts to denature at 55°C and the structure is destroyed by the time the temperature reaches 80°C | Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | the half-life of the enzyme activity at 30°C is about 7 h and the half-life of the enzyme activity at 50°C is 45 min | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Pseudomonas putida |
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