KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
4-n-butylcatechol | 25°C, pH 7.2 | Pseudomonas putida | |
0.0084 | - |
4-methylcatechol | 25°C, pH 7.2 | Pseudomonas putida | |
0.0234 | - |
catechol | 25°C, pH 7.2 | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q842G7 | recombinantly expressed in Escherichia coli | - |
Pseudomonas putida MT4 | Q842G7 | recombinantly expressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.7 | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxybiphenyl + O2 | 1.3% of the activity with 2,3-dihydroxybiphenyl | Pseudomonas putida | ? | - |
? | |
2,3-dihydroxybiphenyl + O2 | 1.3% of the activity with 2,3-dihydroxybiphenyl | Pseudomonas putida MT4 | ? | - |
? | |
3-methylcatechol + O2 | 33% of the activity with 4-chlorocatechol | Pseudomonas putida | ? | - |
? | |
3-methylcatechol + O2 | 33% of the activity with 4-chlorocatechol | Pseudomonas putida MT4 | ? | - |
? | |
4-chlorocatechol + O2 | 39% of the activity with 4-chlorocatechol | Pseudomonas putida | ? | - |
? | |
4-chlorocatechol + O2 | 39% of the activity with 4-chlorocatechol | Pseudomonas putida MT4 | ? | - |
? | |
4-methylcatechol + O2 | activity is 5.72fold higher than with catechol | Pseudomonas putida | ? | - |
? | |
4-n-butylcatechol + O2 | activity is 1.85fold higher than with catechol | Pseudomonas putida | ? | - |
? | |
4-n-heptylcatechol + O2 | 45% of the activity with catechol | Pseudomonas putida | ? | - |
? | |
4-n-hexylcatechol + O2 | 53% of the activity with catechol | Pseudomonas putida | ? | - |
? | |
4-n-nonylcatechol + O2 | 10% of the activity with catechol | Pseudomonas putida | ? | - |
? | |
catechol + O2 | - |
Pseudomonas putida | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | - |
Pseudomonas putida MT4 | 2-hydroxymuconate semialdehyde | - |
? | |
additional information | no activity with 4-tert-butylcatechol | Pseudomonas putida | ? | - |
? | |
additional information | no activity with 4-tert-butylcatechol | Pseudomonas putida MT4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
A23O | - |
Pseudomonas putida |
BupB | - |
Pseudomonas putida |