Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3) | Pseudomonas stutzeri |
Protein Variants | Comment | Organism |
---|---|---|
T249A | reduced kcat and increased Km-values | Pseudomonas stutzeri |
T249G | reduced kcat and increased Km-values | Pseudomonas stutzeri |
T249S | reduced kcat and increased Km-values | Pseudomonas stutzeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
catechol | wildtype | Pseudomonas stutzeri | |
0.0038 | - |
3-methylcatechol | wildtype | Pseudomonas stutzeri | |
0.0055 | - |
3,5-dimethylcatechol | mutant T249A | Pseudomonas stutzeri | |
0.0074 | - |
3,5-dimethylcatechol | mutant T249G | Pseudomonas stutzeri | |
0.0097 | - |
3,5-dimethylcatechol | mutant T249S | Pseudomonas stutzeri | |
0.0115 | - |
3-methylcatechol | mutant T249S | Pseudomonas stutzeri | |
0.0143 | - |
3-methylcatechol | mutant T249A | Pseudomonas stutzeri | |
0.0215 | - |
3,5-dimethylcatechol | wildtype | Pseudomonas stutzeri | |
0.0225 | - |
catechol | mutant T249S | Pseudomonas stutzeri | |
0.0237 | - |
3,5-dimethylcatechol | mutant T249G | Pseudomonas stutzeri | |
0.0266 | - |
3-methylcatechol | mutant T249G | Pseudomonas stutzeri | |
0.037 | - |
catechol | mutant T249A | Pseudomonas stutzeri | |
0.0381 | - |
3,5-dimethylcatechol | mutant T249A | Pseudomonas stutzeri | |
0.0575 | - |
3,5-dimethylcatechol | mutant T249S | Pseudomonas stutzeri | |
0.0636 | - |
catechol | mutant T249G | Pseudomonas stutzeri | |
0.0738 | - |
3,5-dimethylcatechol | wildtype | Pseudomonas stutzeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | Pseudomonas stutzeri | - |
2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | Pseudomonas stutzeri OX1 | - |
2-hydroxymuconate semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas stutzeri | - |
OX1 | - |
Pseudomonas stutzeri OX1 | - |
OX1 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas stutzeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,5-dimethylcatechol + O2 | - |
Pseudomonas stutzeri | 2-hydroxy-3,5-dimethyl-6-oxohexa-2,4-dienoic acid | - |
? | |
3,5-dimethylcatechol + O2 | - |
Pseudomonas stutzeri OX1 | 2-hydroxy-3,5-dimethyl-6-oxohexa-2,4-dienoic acid | - |
? | |
3-methylcatechol + O2 | - |
Pseudomonas stutzeri | 2-hydroxy-6-oxohepta-2,4-dienoic acid | - |
? | |
3-methylcatechol + O2 | - |
Pseudomonas stutzeri OX1 | 2-hydroxy-6-oxohepta-2,4-dienoic acid | - |
? | |
catechol + O2 | - |
Pseudomonas stutzeri | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | - |
Pseudomonas stutzeri OX1 | 2-hydroxymuconate semialdehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C2,3O | - |
Pseudomonas stutzeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
3,5-dimethylcatechol | mutant T249G | Pseudomonas stutzeri | |
0.36 | - |
3,5-dimethylcatechol | wildtype | Pseudomonas stutzeri | |
0.4 | - |
3,5-dimethylcatechol | mutant T249A | Pseudomonas stutzeri | |
0.4 | - |
3,5-dimethylcatechol | mutant T249G | Pseudomonas stutzeri | |
0.66 | - |
3,5-dimethylcatechol | wildtype | Pseudomonas stutzeri | |
0.8 | - |
catechol | mutant T249G | Pseudomonas stutzeri | |
1 | - |
3,5-dimethylcatechol | mutant T249A | Pseudomonas stutzeri | |
1.2 | - |
3,5-dimethylcatechol | mutant T249S | Pseudomonas stutzeri | |
1.3 | - |
catechol | mutant T249A | Pseudomonas stutzeri | |
2.6 | - |
3-methylcatechol | mutant T249G | Pseudomonas stutzeri | |
2.65 | - |
3,5-dimethylcatechol | mutant T249S | Pseudomonas stutzeri | |
6.3 | - |
3-methylcatechol | mutant T249A | Pseudomonas stutzeri | |
7.5 | - |
catechol | mutant T249S | Pseudomonas stutzeri | |
60.5 | - |
3-methylcatechol | mutant T249S | Pseudomonas stutzeri | |
118 | - |
3-methylcatechol | wildtype | Pseudomonas stutzeri | |
180 | - |
catechol | wildtype | Pseudomonas stutzeri |