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Literature summary for 1.13.11.2 extracted from

  • Siani, L.; Viggiani, A.; Notomista, E.; Pezzella, A.; Di Donato, A.
    The role of residue Thr249 in modulating the catalytic efficiency and substrate specificity of catechol-2,3-dioxygenase from Pseudomonas stutzeri OX1 (2006), FEBS J., 273, 2963-2976.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Pseudomonas stutzeri

Protein Variants

Protein Variants Comment Organism
T249A reduced kcat and increased Km-values Pseudomonas stutzeri
T249G reduced kcat and increased Km-values Pseudomonas stutzeri
T249S reduced kcat and increased Km-values Pseudomonas stutzeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
catechol wildtype Pseudomonas stutzeri
0.0038
-
3-methylcatechol wildtype Pseudomonas stutzeri
0.0055
-
3,5-dimethylcatechol mutant T249A Pseudomonas stutzeri
0.0074
-
3,5-dimethylcatechol mutant T249G Pseudomonas stutzeri
0.0097
-
3,5-dimethylcatechol mutant T249S Pseudomonas stutzeri
0.0115
-
3-methylcatechol mutant T249S Pseudomonas stutzeri
0.0143
-
3-methylcatechol mutant T249A Pseudomonas stutzeri
0.0215
-
3,5-dimethylcatechol wildtype Pseudomonas stutzeri
0.0225
-
catechol mutant T249S Pseudomonas stutzeri
0.0237
-
3,5-dimethylcatechol mutant T249G Pseudomonas stutzeri
0.0266
-
3-methylcatechol mutant T249G Pseudomonas stutzeri
0.037
-
catechol mutant T249A Pseudomonas stutzeri
0.0381
-
3,5-dimethylcatechol mutant T249A Pseudomonas stutzeri
0.0575
-
3,5-dimethylcatechol mutant T249S Pseudomonas stutzeri
0.0636
-
catechol mutant T249G Pseudomonas stutzeri
0.0738
-
3,5-dimethylcatechol wildtype Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
catechol + O2 Pseudomonas stutzeri
-
2-hydroxymuconate semialdehyde
-
?
catechol + O2 Pseudomonas stutzeri OX1
-
2-hydroxymuconate semialdehyde
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas stutzeri
-
OX1
-
Pseudomonas stutzeri OX1
-
OX1
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas stutzeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,5-dimethylcatechol + O2
-
Pseudomonas stutzeri 2-hydroxy-3,5-dimethyl-6-oxohexa-2,4-dienoic acid
-
?
3,5-dimethylcatechol + O2
-
Pseudomonas stutzeri OX1 2-hydroxy-3,5-dimethyl-6-oxohexa-2,4-dienoic acid
-
?
3-methylcatechol + O2
-
Pseudomonas stutzeri 2-hydroxy-6-oxohepta-2,4-dienoic acid
-
?
3-methylcatechol + O2
-
Pseudomonas stutzeri OX1 2-hydroxy-6-oxohepta-2,4-dienoic acid
-
?
catechol + O2
-
Pseudomonas stutzeri 2-hydroxymuconate semialdehyde
-
?
catechol + O2
-
Pseudomonas stutzeri OX1 2-hydroxymuconate semialdehyde
-
?

Synonyms

Synonyms Comment Organism
C2,3O
-
Pseudomonas stutzeri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.23
-
3,5-dimethylcatechol mutant T249G Pseudomonas stutzeri
0.36
-
3,5-dimethylcatechol wildtype Pseudomonas stutzeri
0.4
-
3,5-dimethylcatechol mutant T249A Pseudomonas stutzeri
0.4
-
3,5-dimethylcatechol mutant T249G Pseudomonas stutzeri
0.66
-
3,5-dimethylcatechol wildtype Pseudomonas stutzeri
0.8
-
catechol mutant T249G Pseudomonas stutzeri
1
-
3,5-dimethylcatechol mutant T249A Pseudomonas stutzeri
1.2
-
3,5-dimethylcatechol mutant T249S Pseudomonas stutzeri
1.3
-
catechol mutant T249A Pseudomonas stutzeri
2.6
-
3-methylcatechol mutant T249G Pseudomonas stutzeri
2.65
-
3,5-dimethylcatechol mutant T249S Pseudomonas stutzeri
6.3
-
3-methylcatechol mutant T249A Pseudomonas stutzeri
7.5
-
catechol mutant T249S Pseudomonas stutzeri
60.5
-
3-methylcatechol mutant T249S Pseudomonas stutzeri
118
-
3-methylcatechol wildtype Pseudomonas stutzeri
180
-
catechol wildtype Pseudomonas stutzeri