Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli W3110 | Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(1R,2R)-trans-1,2-cyclohexanediol | - |
Pseudomonas putida | |
(1S,2S)-trans-1,2-cyclohexanediol | - |
Pseudomonas putida | |
1-naphthoquinoline | - |
Pseudomonas putida | |
4-Methyl-2-nitrophenol | - |
Pseudomonas putida | |
acetone | - |
Pseudomonas putida | |
benzoate | - |
Pseudomonas putida | |
m-fluorophenol | - |
Pseudomonas putida | |
m-Hydroxybenzoate | - |
Pseudomonas putida | |
m-Nitrophenol | - |
Pseudomonas putida | |
nitrobenzene | - |
Pseudomonas putida | |
o-aminophenol | - |
Pseudomonas putida | |
o-fluorophenol | - |
Pseudomonas putida | |
o-Nitrophenol | - |
Pseudomonas putida | |
p-fluorophenol | - |
Pseudomonas putida | |
p-nitrophenol | - |
Pseudomonas putida | |
phenol | - |
Pseudomonas putida | |
salicylate | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00102 | - |
4-Chlorocatechol | - |
Pseudomonas putida | |
0.00129 | - |
4-methylcatechol | - |
Pseudomonas putida | |
0.00164 | - |
3-methylcatechol | - |
Pseudomonas putida | |
0.00187 | - |
catechol | - |
Pseudomonas putida | |
0.00187 | - |
3,5-dichlorocatechol | - |
Pseudomonas putida | |
0.00204 | - |
3-methoxycatechol | - |
Pseudomonas putida | |
0.0228 | - |
Protocatechualdehyde | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 3.7 mol of iron per mol of enzyme or 0.93 mol per mol of the subunit | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
4 * 35000, SDS-PAGE, 4 * 35195, MS, 4 * 35156 based on DNA sequence | Pseudomonas putida |
35156 | - |
4 * 35000, SDS-PAGE, 4 * 35195, MS, 4 * 35156 based on DNA sequence | Pseudomonas putida |
35195 | - |
4 * 35000, SDS-PAGE, 4 * 35195, MS, 4 * 35156 based on DNA sequence | Pseudomonas putida |
136000 | 144000 | light scattering method | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
mt-2 paW1, ATCC 23973 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
536 | - |
crystallized enzyme | Pseudomonas putida |
Storage Stability | Organism |
---|---|
0°C, 50 mM Tris-acetone buffer, pH 7.5, the inactivation rate with decreasing enzyme concentration | Pseudomonas putida |
4°C, Tris-acetone buffer, crystalline enzyme was stable for at most six months | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,5-dichlorocatechol + O2 | - |
Pseudomonas putida | ? | - |
? | |
3-methoxycatechol + O2 | - |
Pseudomonas putida | 2-hydroxy-3-methoxy-6-oxohexa-2,4-dienoate | - |
? | |
3-methylcatechol + O2 | - |
Pseudomonas putida | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
4-chlorocatechol + O2 | - |
Pseudomonas putida | 5-chloro-2-hydroxymuconic semialdehyde | - |
? | |
4-methylcatechol + O2 | - |
Pseudomonas putida | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
catechol + O2 | - |
Pseudomonas putida | 2-hydroxymuconate semialdehyde | - |
? | |
protocatechualdehyde + O2 | weak | Pseudomonas putida | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 35000, SDS-PAGE, 4 * 35195, MS, 4 * 35156 based on DNA sequence | Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00056 | - |
1-naphthoquinoline | - |
Pseudomonas putida | |
0.0049 | - |
o-aminophenol | - |
Pseudomonas putida | |
0.0093 | - |
o-Nitrophenol | - |
Pseudomonas putida | |
0.042 | - |
m-Nitrophenol | - |
Pseudomonas putida | |
0.056 | - |
o-fluorophenol | - |
Pseudomonas putida | |
0.111 | - |
p-nitrophenol | - |
Pseudomonas putida | |
0.126 | - |
4-Methyl-2-nitrophenol | - |
Pseudomonas putida | |
0.205 | - |
nitrobenzene | - |
Pseudomonas putida | |
0.311 | - |
m-fluorophenol | - |
Pseudomonas putida | |
0.452 | - |
p-fluorophenol | - |
Pseudomonas putida | |
1.7 | - |
phenol | - |
Pseudomonas putida | |
4.9 | - |
benzoate | - |
Pseudomonas putida | |
5.2 | - |
salicylate | - |
Pseudomonas putida | |
13 | - |
acetone | - |
Pseudomonas putida | |
14.7 | - |
m-Hydroxybenzoate | - |
Pseudomonas putida | |
34 | - |
(1R,2R)-trans-1,2-cyclohexanediol | - |
Pseudomonas putida | |
43 | - |
(1S,2S)-trans-1,2-cyclohexanediol | - |
Pseudomonas putida |