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Literature summary for 1.13.11.2 extracted from

  • Kobayashi, T.; Ishida, T.; Horiike, K.; Takahara, Y.; Numao, N.; Nakazawa, A.; Nakazawa, T.; Nozaki, M.
    Overexpression of Pseudomonas putida catechol 2,3-dioxygenase with high specific activity by genetically engineered Escherichia coli (1995), J. Biochem., 117, 614-622.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli W3110 Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
(1R,2R)-trans-1,2-cyclohexanediol
-
Pseudomonas putida
(1S,2S)-trans-1,2-cyclohexanediol
-
Pseudomonas putida
1-naphthoquinoline
-
Pseudomonas putida
4-Methyl-2-nitrophenol
-
Pseudomonas putida
acetone
-
Pseudomonas putida
benzoate
-
Pseudomonas putida
m-fluorophenol
-
Pseudomonas putida
m-Hydroxybenzoate
-
Pseudomonas putida
m-Nitrophenol
-
Pseudomonas putida
nitrobenzene
-
Pseudomonas putida
o-aminophenol
-
Pseudomonas putida
o-fluorophenol
-
Pseudomonas putida
o-Nitrophenol
-
Pseudomonas putida
p-fluorophenol
-
Pseudomonas putida
p-nitrophenol
-
Pseudomonas putida
phenol
-
Pseudomonas putida
salicylate
-
Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00102
-
4-Chlorocatechol
-
Pseudomonas putida
0.00129
-
4-methylcatechol
-
Pseudomonas putida
0.00164
-
3-methylcatechol
-
Pseudomonas putida
0.00187
-
catechol
-
Pseudomonas putida
0.00187
-
3,5-dichlorocatechol
-
Pseudomonas putida
0.00204
-
3-methoxycatechol
-
Pseudomonas putida
0.0228
-
Protocatechualdehyde
-
Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 3.7 mol of iron per mol of enzyme or 0.93 mol per mol of the subunit Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
4 * 35000, SDS-PAGE, 4 * 35195, MS, 4 * 35156 based on DNA sequence Pseudomonas putida
35156
-
4 * 35000, SDS-PAGE, 4 * 35195, MS, 4 * 35156 based on DNA sequence Pseudomonas putida
35195
-
4 * 35000, SDS-PAGE, 4 * 35195, MS, 4 * 35156 based on DNA sequence Pseudomonas putida
136000 144000 light scattering method Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
mt-2 paW1, ATCC 23973
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
536
-
crystallized enzyme Pseudomonas putida

Storage Stability

Storage Stability Organism
0°C, 50 mM Tris-acetone buffer, pH 7.5, the inactivation rate with decreasing enzyme concentration Pseudomonas putida
4°C, Tris-acetone buffer, crystalline enzyme was stable for at most six months Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,5-dichlorocatechol + O2
-
Pseudomonas putida ?
-
?
3-methoxycatechol + O2
-
Pseudomonas putida 2-hydroxy-3-methoxy-6-oxohexa-2,4-dienoate
-
?
3-methylcatechol + O2
-
Pseudomonas putida 2-hydroxy-6-oxohepta-2,4-dienoate
-
?
4-chlorocatechol + O2
-
Pseudomonas putida 5-chloro-2-hydroxymuconic semialdehyde
-
?
4-methylcatechol + O2
-
Pseudomonas putida 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
?
catechol + O2
-
Pseudomonas putida 2-hydroxymuconate semialdehyde
-
?
protocatechualdehyde + O2 weak Pseudomonas putida ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 35000, SDS-PAGE, 4 * 35195, MS, 4 * 35156 based on DNA sequence Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00056
-
1-naphthoquinoline
-
Pseudomonas putida
0.0049
-
o-aminophenol
-
Pseudomonas putida
0.0093
-
o-Nitrophenol
-
Pseudomonas putida
0.042
-
m-Nitrophenol
-
Pseudomonas putida
0.056
-
o-fluorophenol
-
Pseudomonas putida
0.111
-
p-nitrophenol
-
Pseudomonas putida
0.126
-
4-Methyl-2-nitrophenol
-
Pseudomonas putida
0.205
-
nitrobenzene
-
Pseudomonas putida
0.311
-
m-fluorophenol
-
Pseudomonas putida
0.452
-
p-fluorophenol
-
Pseudomonas putida
1.7
-
phenol
-
Pseudomonas putida
4.9
-
benzoate
-
Pseudomonas putida
5.2
-
salicylate
-
Pseudomonas putida
13
-
acetone
-
Pseudomonas putida
14.7
-
m-Hydroxybenzoate
-
Pseudomonas putida
34
-
(1R,2R)-trans-1,2-cyclohexanediol
-
Pseudomonas putida
43
-
(1S,2S)-trans-1,2-cyclohexanediol
-
Pseudomonas putida