General Stability | Organism |
---|---|
immobilization enhances stability against inactivation by heat, acid or alkaline pH and various denaturing agents | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pseudomonas putida | |
0.063 | - |
O2 | immobilized enzyme | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Oxidation Stability | Organism |
---|---|
like native enzyme the immobilized enzyme is rapidly inactivated by oxidants such as O2 or H2O2 | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
46 | - |
immobilized enzyme | Pseudomonas putida |
270 | - |
free enzyme | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-chlorocatechol + O2 | - |
Pseudomonas putida | 2-chloro-2-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
3-hydroxycatechol + O2 | - |
Pseudomonas putida | 3-hydroxymuconic acid | - |
? | |
3-methylcatechol + O2 | - |
Pseudomonas putida | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | highly specific for | Pseudomonas putida | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
catechol + O2 | - |
Pseudomonas putida | 2-hydroxymuconate semialdehyde | - |
? | |
protocatechualdehyde + O2 | - |
Pseudomonas putida | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Pseudomonas putida |