Protein Variants | Comment | Organism |
---|---|---|
C161Y | mutation reported in ethylmalonic encephalopathy patients, drastic reduction in iron content | Homo sapiens |
L55P | mutation reported in ethylmalonic encephalopathy patients, drastic reduction in iron content | Homo sapiens |
R163W | mutation reported in ethylmalonic encephalopathy patients | Homo sapiens |
T136A | mutation reported in ethylmalonic encephalopathy patients | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
gamma-glutamyl-homocysteinyl-glycine | mechanism-based inhibitor, accepted as an alternative substrate and converted to glutamyl-homocysteinyl-SO2H, which fails to undergo the final hydrolysis step in the catalytic cycle, leading to inhibition. Maximum inhibition to 30% of initial activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
S-sulfanylglutathione | wild-type, pH 7.4, 22°C | Homo sapiens | |
0.73 | - |
S-sulfanylglutathione | mutant R163W, pH 7.4, 22°C | Homo sapiens | |
0.86 | - |
S-sulfanylglutathione | mutant L55P, pH 7.4, 22°C | Homo sapiens | |
1.1 | - |
S-sulfanylglutathione | mutant T136A, pH 7.4, 22°C | Homo sapiens | |
1.2 | - |
S-sulfanylglutathione | mutant C161Y, pH 7.4, 22°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron content of wild-type is 0.8 mol/mol of enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O95571 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
113 | - |
wild-type, pH 7.4, 22°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gamma-glutamyl-homocysteinyl-glycine + O2 | mechanism-based inhibitor, accepted as an alternative substrate and converted to glutamyl-homocysteinyl-SO2H | Homo sapiens | gamma-glutamyl-homocysteinyl-SO2H + ? | - |
? | |
S-sulfanylglutathione + O2 + H2O | - |
Homo sapiens | glutathione + sulfite + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ETHE1 | - |
Homo sapiens |
PDO | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
mutant C161Y, melting temperature | Homo sapiens |
44 | - |
mutant T136A, melting temperature | Homo sapiens |
47 | - |
mutant L55P, melting temperature | Homo sapiens |
53 | - |
mutant R163W, melting temperature | Homo sapiens |
64 | - |
wild-type, melting temperature | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
S-sulfanylglutathione | mutant T136A, pH 7.4, 22°C | Homo sapiens | |
2.6 | - |
S-sulfanylglutathione | mutant C161Y, pH 7.4, 22°C | Homo sapiens | |
2.8 | - |
S-sulfanylglutathione | mutant L55P, pH 7.4, 22°C | Homo sapiens | |
5.6 | - |
S-sulfanylglutathione | mutant R163W, pH 7.4, 22°C | Homo sapiens | |
47 | - |
S-sulfanylglutathione | wild-type, pH 7.4, 22°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
S-sulfanylglutathione | mutant T136A, pH 7.4, 22°C | Homo sapiens | |
2.2 | - |
S-sulfanylglutathione | mutant C161Y, pH 7.4, 22°C | Homo sapiens | |
3.3 | - |
S-sulfanylglutathione | mutant L55P, pH 7.4, 22°C | Homo sapiens | |
7.6 | - |
S-sulfanylglutathione | mutant R163W, pH 7.4, 22°C | Homo sapiens | |
140 | - |
S-sulfanylglutathione | wild-type, pH 7.4, 22°C | Homo sapiens |