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Literature summary for 1.13.11.18 extracted from

  • Kabil, O.; Motl, N.; Strack, M.; Seravalli, J.; Metzler-Nolte, N.; Banerjee, R.
    Mechanism-based inhibition of human persulfide dioxygenase by gamma-glutamyl-homocysteinyl-glycine (2018), J. Biol. Chem., 293, 12429-12439 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.18

Protein Variants

Protein Variants Comment Organism
C161Y mutation reported in ethylmalonic encephalopathy patients, drastic reduction in iron content Homo sapiens
L55P mutation reported in ethylmalonic encephalopathy patients, drastic reduction in iron content Homo sapiens
R163W mutation reported in ethylmalonic encephalopathy patients Homo sapiens
T136A mutation reported in ethylmalonic encephalopathy patients Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
gamma-glutamyl-homocysteinyl-glycine mechanism-based inhibitor, accepted as an alternative substrate and converted to glutamyl-homocysteinyl-SO2H, which fails to undergo the final hydrolysis step in the catalytic cycle, leading to inhibition. Maximum inhibition to 30% of initial activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
 S-sulfanylglutathione wild-type, pH 7.4, 22°C Homo sapiens
0.73
-
 S-sulfanylglutathione mutant R163W, pH 7.4, 22°C Homo sapiens
0.86
-
 S-sulfanylglutathione mutant L55P, pH 7.4, 22°C Homo sapiens
1.1
-
 S-sulfanylglutathione mutant T136A, pH 7.4, 22°C Homo sapiens
1.2
-
 S-sulfanylglutathione mutant C161Y, pH 7.4, 22°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron content of wild-type is 0.8 mol/mol of enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O95571
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
113
-
 wild-type, pH 7.4, 22°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gamma-glutamyl-homocysteinyl-glycine + O2 mechanism-based inhibitor, accepted as an alternative substrate and converted to glutamyl-homocysteinyl-SO2H Homo sapiens gamma-glutamyl-homocysteinyl-SO2H + ?
-
 ?
S-sulfanylglutathione + O2 + H2O
-
 Homo sapiens glutathione + sulfite + 2 H+
-
 ?

Synonyms

Synonyms Comment Organism
ETHE1
-
 Homo sapiens
PDO
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
38
-
 mutant C161Y, melting temperature Homo sapiens
44
-
 mutant T136A, melting temperature Homo sapiens
47
-
 mutant L55P, melting temperature Homo sapiens
53
-
 mutant R163W, melting temperature Homo sapiens
64
-
 wild-type, melting temperature Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 S-sulfanylglutathione mutant T136A, pH 7.4, 22°C Homo sapiens
2.6
-
 S-sulfanylglutathione mutant C161Y, pH 7.4, 22°C Homo sapiens
2.8
-
 S-sulfanylglutathione mutant L55P, pH 7.4, 22°C Homo sapiens
5.6
-
 S-sulfanylglutathione mutant R163W, pH 7.4, 22°C Homo sapiens
47
-
 S-sulfanylglutathione wild-type, pH 7.4, 22°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.7
-
 S-sulfanylglutathione mutant T136A, pH 7.4, 22°C Homo sapiens
2.2
-
 S-sulfanylglutathione mutant C161Y, pH 7.4, 22°C Homo sapiens
3.3
-
 S-sulfanylglutathione mutant L55P, pH 7.4, 22°C Homo sapiens
7.6
-
 S-sulfanylglutathione mutant R163W, pH 7.4, 22°C Homo sapiens
140
-
 S-sulfanylglutathione wild-type, pH 7.4, 22°C Homo sapiens