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Literature summary for 1.13.11.18 extracted from

  • Ruehl, P.; Haas, P.; Seipel, D.; Becker, J.; Kletzin, A.
    Persulfide dioxygenase from Acidithiobacillus caldus variable roles of cysteine residues and hydrogen bond networks of the active site (2018), Front. Microbiol., 9, 1610 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Acidithiobacillus caldus

Protein Variants

Protein Variants Comment Organism
D130A mutation in putative iron ligand, 0.5-2% residual activity Acidithiobacillus caldus
D130E mutation in putative iron ligand, 0.5-2% residual activity Acidithiobacillus caldus
D130H mutation in putative iron ligand, 0.5-2% residual activity Acidithiobacillus caldus
D141A inactive concomitant to a decrease of melting point by 3-8 K Acidithiobacillus caldus
H113A mutation in putative iron ligand, inactive Acidithiobacillus caldus
H113G mutation in putative iron ligand, inactive Acidithiobacillus caldus
H171A inactive concomitant to a decrease of melting point by 3-8 K Acidithiobacillus caldus
H57A mutation in putative iron ligand, 6% residual activity Acidithiobacillus caldus
H57G mutation in putative iron ligand, inactive Acidithiobacillus caldus
additional information residues located in the predicted GSH/GSSH binding site and in the central hydrogen bond networks including the iron ligands are essential for activity Acidithiobacillus caldus
R139A inactive concomitant to a decrease of melting point by 3-8 K Acidithiobacillus caldus

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
Acidithiobacillus caldus
N-ethylmaleimide
-
Acidithiobacillus caldus
tris(2-carboxyethyl)phosphine
-
Acidithiobacillus caldus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
glutathione pH 7.5, 40°C Acidithiobacillus caldus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 0.77 mol per mol of subunit Acidithiobacillus caldus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
PAGE Acidithiobacillus caldus
99400
-
gel filtration Acidithiobacillus caldus

Organism

Organism UniProt Comment Textmining
Acidithiobacillus caldus A0A2P9DTT9
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information residues C87 and C224 form a disulfide bridge Acidithiobacillus caldus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
111.5
-
40°C, pH 7.5 Acidithiobacillus caldus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + sulfite + 2 H+
-
Acidithiobacillus caldus S-sulfanylglutathione + O2 + H2O
-
r
S-sulfanylglutathione + O2 + H2O
-
Acidithiobacillus caldus glutathione + sulfite + 2 H+
-
r

Subunits

Subunits Comment Organism
dimer and tetramer, 4 * 27000, SDS-PAGE Acidithiobacillus caldus
tetramer and dimer, 4 * 27000, SDS-PAGE Acidithiobacillus caldus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
Acidithiobacillus caldus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
63
-
melting temperature Acidithiobacillus caldus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
181
-
glutathione pH 7.5, 40°C Acidithiobacillus caldus
198
-
S-sulfanylglutathione sigmoidal curve with Hill coefficient 2.3, pH 7.5, 40°C Acidithiobacillus caldus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
361
-
glutathione pH 7.5, 40°C Acidithiobacillus caldus