BRENDA - Enzyme Database show
show all sequences of 1.13.11.18

The initial product and properties of the sulfur-oxidizing enzyme of thiobacilli

Suzuki, I.; Silver, M.; Biochim. Biophys. Acta 122, 22-33 (1966)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
1,10-phenanthroline
similar stimulating effect as 2,2' dipyridyl
Thiobacillus thioparus
2,2'-dipyridyl
stimulation at low concentrations e.g. 0.1 mM, may protect glutathione from destruction
Thiobacillus thioparus
catalase
-
Thiobacillus thioparus
General Stability
General Stability
Organism
2,2'-dipyridyl stabilizes cofactor glutathione, activity is lost upon freezing and thawing
Thiobacillus thioparus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Co2+
0.1 mM
Thiobacillus thioparus
Cu2+
0.1 mM
Thiobacillus thioparus
diethyldithiocarbamate
-
Thiobacillus thioparus
EDTA
-
Thiobacillus thioparus
FAD
-
Thiobacillus thioparus
Fe2+
-
Thiobacillus thioparus
Fe3+
-
Thiobacillus thioparus
FMN
-
Thiobacillus thioparus
H2O2
strong inhibition, reversible by subsequent addition of catalase and glutathione
Thiobacillus thioparus
Zn2+
0.1 mM
Thiobacillus thioparus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3
-
glutathione
-
Thiobacillus thioparus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Non-heme iron
-
Acidithiobacillus thiooxidans
Non-heme iron
0.000087 mmol of iron per mg of enzyme
Thiobacillus thioparus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sulfur + O2 + H2O
Thiobacillus thioparus
-
sulfite
-
Thiobacillus thioparus
ir
sulfur + O2 + H2O
Acidithiobacillus thiooxidans
-
sulfite
-
Acidithiobacillus thiooxidans
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acidithiobacillus thiooxidans
-
-
-
Thiobacillus thioparus
-
-
-
Purification (Commentary)
Commentary
Organism
30% ethanol, DEAE-cellulose
Thiobacillus thioparus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.25
-
-
Thiobacillus thioparus
Storage Stability
Storage Stability
Organism
-20°C, DEAE-cellulose fraction, 7 d, almost complete loss of activity
Thiobacillus thioparus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sulfur + O2 + H2O
-
439598
Thiobacillus thioparus
sulfite
-
439598
Thiobacillus thioparus
ir
sulfur + O2 + H2O
-
439598
Acidithiobacillus thiooxidans
sulfite
-
439598
Acidithiobacillus thiooxidans
ir
sulfur + O2 + H2O
-
439598
Thiobacillus thioparus
sulfite
S2O32- may be formed through a secondary, non-enzymatic reaction
439598
Thiobacillus thioparus
ir
sulfur + O2 + H2O
-
439598
Acidithiobacillus thiooxidans
sulfite
S2O32- may be formed through a secondary, non-enzymatic reaction
439598
Acidithiobacillus thiooxidans
ir
Cofactor
Cofactor
Commentary
Organism
Structure
glutathione
-
Acidithiobacillus thiooxidans
glutathione
-
Thiobacillus thioparus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
1,10-phenanthroline
similar stimulating effect as 2,2' dipyridyl
Thiobacillus thioparus
2,2'-dipyridyl
stimulation at low concentrations e.g. 0.1 mM, may protect glutathione from destruction
Thiobacillus thioparus
catalase
-
Thiobacillus thioparus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
glutathione
-
Acidithiobacillus thiooxidans
glutathione
-
Thiobacillus thioparus
General Stability (protein specific)
General Stability
Organism
2,2'-dipyridyl stabilizes cofactor glutathione, activity is lost upon freezing and thawing
Thiobacillus thioparus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Co2+
0.1 mM
Thiobacillus thioparus
Cu2+
0.1 mM
Thiobacillus thioparus
diethyldithiocarbamate
-
Thiobacillus thioparus
EDTA
-
Thiobacillus thioparus
FAD
-
Thiobacillus thioparus
Fe2+
-
Thiobacillus thioparus
Fe3+
-
Thiobacillus thioparus
FMN
-
Thiobacillus thioparus
H2O2
strong inhibition, reversible by subsequent addition of catalase and glutathione
Thiobacillus thioparus
Zn2+
0.1 mM
Thiobacillus thioparus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3
-
glutathione
-
Thiobacillus thioparus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Non-heme iron
-
Acidithiobacillus thiooxidans
Non-heme iron
0.000087 mmol of iron per mg of enzyme
Thiobacillus thioparus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sulfur + O2 + H2O
Thiobacillus thioparus
-
sulfite
-
Thiobacillus thioparus
ir
sulfur + O2 + H2O
Acidithiobacillus thiooxidans
-
sulfite
-
Acidithiobacillus thiooxidans
ir
Purification (Commentary) (protein specific)
Commentary
Organism
30% ethanol, DEAE-cellulose
Thiobacillus thioparus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.25
-
-
Thiobacillus thioparus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, DEAE-cellulose fraction, 7 d, almost complete loss of activity
Thiobacillus thioparus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sulfur + O2 + H2O
-
439598
Thiobacillus thioparus
sulfite
-
439598
Thiobacillus thioparus
ir
sulfur + O2 + H2O
-
439598
Acidithiobacillus thiooxidans
sulfite
-
439598
Acidithiobacillus thiooxidans
ir
sulfur + O2 + H2O
-
439598
Thiobacillus thioparus
sulfite
S2O32- may be formed through a secondary, non-enzymatic reaction
439598
Thiobacillus thioparus
ir
sulfur + O2 + H2O
-
439598
Acidithiobacillus thiooxidans
sulfite
S2O32- may be formed through a secondary, non-enzymatic reaction
439598
Acidithiobacillus thiooxidans
ir
Other publictions for EC 1.13.11.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742906
Motl
Structural and biochemical an ...
Paraburkholderia phytofirmans
J. Biol. Chem.
292
14026-14038
2017
3
-
1
1
1
-
2
4
-
1
1
2
-
6
-
-
1
-
-
-
-
-
3
1
1
-
1
3
1
-
-
-
-
-
-
3
-
1
-
1
1
-
-
2
-
4
-
1
1
2
-
-
-
1
-
-
-
-
3
1
1
-
1
3
1
-
-
-
-
3
3
-
3
3
742616
Zhang
Expression characteristics of ...
Urechis unicinctus
Gene
593
334-341
2016
1
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
8
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
1
-
-
-
1
-
-
-
1
1
1
1
-
-
741915
Shen
Staphylococcus aureus CstB is ...
Staphylococcus aureus
Biochemistry
54
4542-4554
2015
-
-
1
-
4
-
-
4
-
1
2
-
-
4
-
-
-
-
1
-
-
-
5
2
1
-
-
4
1
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
4
-
1
2
-
-
-
-
-
1
-
-
-
5
2
1
-
-
4
1
-
-
-
1
3
3
1
4
4
741742
Wang
Identification and characteri ...
Acidithiobacillus caldus, Acidithiobacillus caldus MTH-04, Acidithiobacillus ferrooxidans
Appl. Microbiol. Biotechnol.
98
7511-7522
2014
1
-
2
-
1
-
23
-
-
2
-
-
-
8
-
-
-
-
-
-
2
-
-
1
2
1
-
-
2
2
-
-
-
1
-
1
-
2
-
-
1
-
-
23
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
1
2
1
-
-
2
2
-
1
-
1
1
-
-
-
726227
Holdorf
Arabidopsis ETHE1 encodes a su ...
Arabidopsis thaliana
Plant Physiol.
160
226-236
2012
-
-
1
-
1
-
-
-
2
-
-
1
-
3
-
-
1
-
-
4
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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1
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-
-
-
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2
-
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1
-
-
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1
-
4
1
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
713090
Viscomi
Combined treatment with oral m ...
Homo sapiens, Mus musculus
Nat. Med.
16
869-871
2010
-
2
-
-
1
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
657575
Krasil'nikova
-
Sulfur-metabolizing enzymes in ...
Sulfobacillus sibiricus
Appl. Biochem. Microbiol.
40
53-56
2004
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
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1
-
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-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658665
Sun
Purification and properties of ...
Acidianus sp., Acidianus sp. S5
Extremophiles
7
131-134
2003
-
-
1
-
-
-
-
-
-
-
1
2
-
2
-
-
1
-
-
-
1
-
4
-
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
1
-
4
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
659877
Rohwerder
The sulfane sulfur of persulfi ...
Acidiphilium acidophilum, Acidithiobacillus ferrooxidans, Acidithiobacillus ferrooxidans R1, Acidithiobacillus thiooxidans
Microbiology
149
1699-1710
2003
1
-
-
-
-
-
-
-
3
-
2
4
-
9
-
-
-
-
-
-
-
-
8
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
2
4
-
-
-
-
-
-
-
-
8
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439599
Nogami
-
Inhibition of sulfur oxidizing ...
Acidithiobacillus thiooxidans, Acidithiobacillus thiooxidans NB1-3
Biosci. Biotechnol. Biochem.
61
1373-1375
1997
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
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2
-
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-
-
-
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-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439595
Emmel
-
Evidence for the existence of ...
Acidianus brierleyi
J. Gen. Microbiol.
132
3415-3420
1986
-
-
-
-
-
-
-
1
-
-
2
1
-
1
-
-
1
-
-
-
1
-
2
1
1
1
-
-
1
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
1
-
-
2
1
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-
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1
-
-
1
-
2
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
439596
Justin
-
Metabolic changes in Thiobacil ...
Thiobacillus denitrificans
J. Gen. Microbiol.
107
131-137
1978
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
1
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1
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2
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1
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-
-
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-
-
-
-
-
-
-
-
-
-
394075
Oh
-
Resolution of a membrane-assoc ...
Starkeya novella
J. Gen. Microbiol.
99
413-423
1977
-
-
-
-
-
-
-
-
-
-
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1
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1
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1
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1
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1
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-
-
-
-
-
-
439598
Suzuki
The initial product and proper ...
Acidithiobacillus thiooxidans, Thiobacillus thioparus
Biochim. Biophys. Acta
122
22-33
1966
3
-
-
-
-
1
10
1
-
2
-
2
-
2
-
-
1
-
-
-
1
1
4
-
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-
-
-
-
-
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2
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3
-
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2
-
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1
-
10
-
1
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2
-
2
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1
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1
1
4
-
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