Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | similar stimulating effect as 2,2' dipyridyl | Thiobacillus thioparus | |
2,2'-dipyridyl | stimulation at low concentrations e.g. 0.1 mM, may protect glutathione from destruction | Thiobacillus thioparus | |
catalase | - |
Thiobacillus thioparus |
General Stability | Organism |
---|---|
2,2'-dipyridyl stabilizes cofactor glutathione, activity is lost upon freezing and thawing | Thiobacillus thioparus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.1 mM | Thiobacillus thioparus | |
Cu2+ | 0.1 mM | Thiobacillus thioparus | |
diethyldithiocarbamate | - |
Thiobacillus thioparus | |
EDTA | - |
Thiobacillus thioparus | |
FAD | - |
Thiobacillus thioparus | |
Fe2+ | - |
Thiobacillus thioparus | |
Fe3+ | - |
Thiobacillus thioparus | |
FMN | - |
Thiobacillus thioparus | |
H2O2 | strong inhibition, reversible by subsequent addition of catalase and glutathione | Thiobacillus thioparus | |
Zn2+ | 0.1 mM | Thiobacillus thioparus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.3 | - |
glutathione | - |
Thiobacillus thioparus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
non-heme iron | - |
Acidithiobacillus thiooxidans | |
non-heme iron | 0.000087 mmol of iron per mg of enzyme | Thiobacillus thioparus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfur + O2 + H2O | Thiobacillus thioparus | - |
sulfite | - |
ir | |
sulfur + O2 + H2O | Acidithiobacillus thiooxidans | - |
sulfite | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus thiooxidans | - |
- |
- |
Thiobacillus thioparus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
30% ethanol, DEAE-cellulose | Thiobacillus thioparus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.25 | - |
- |
Thiobacillus thioparus |
Storage Stability | Organism |
---|---|
-20°C, DEAE-cellulose fraction, 7 d, almost complete loss of activity | Thiobacillus thioparus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + sulfite + 2 H+ | - |
Thiobacillus thioparus | S-sulfanylglutathione + O2 + H2O | - |
r | |
sulfur + O2 + H2O | - |
Thiobacillus thioparus | sulfite | - |
ir | |
sulfur + O2 + H2O | - |
Acidithiobacillus thiooxidans | sulfite | - |
ir | |
sulfur + O2 + H2O | - |
Thiobacillus thioparus | sulfite | S2O32- may be formed through a secondary, non-enzymatic reaction | ir | |
sulfur + O2 + H2O | - |
Acidithiobacillus thiooxidans | sulfite | S2O32- may be formed through a secondary, non-enzymatic reaction | ir |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
glutathione | - |
Thiobacillus thioparus | |
glutathione | - |
Acidithiobacillus thiooxidans |