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Literature summary for 1.13.11.17 extracted from

  • Pundir, C.S.; Garg, G.K.; Rathore, V.S.
    Purification and properties of indole 2,3-dioxygenase from maize leaves (1984), Phytochemistry, 23, 2423-2427.
No PubMed abstract available

General Stability

General Stability Organism
pH 7, 0.02 M sodium phosphate buffer, 0.1 mM EDTA, 6 h in the cold, slight loss of activity Zea mays

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 0.5 mM, 80% inhibition, Cu2+ protects Zea mays
Cys 0.5 mM, 78% inhibition, Cu2+ protects Zea mays
diethyldithiocarbamate reversed by Cu2+ Zea mays
Dithionite 1 mM, almost complete inhibition Zea mays
Fe2+ 0.5 mM, slight inhibition Zea mays
Fe3+ 0.5 mM, slight inhibition Zea mays
GSH 0.5 mM, 81% inhibition, Cu2+ protects Zea mays
Hg2+ 0.5 mM, slight inhibition Zea mays
NEM 0.5 mM, 34% inhibition Zea mays
Salicylaldoxime 0.5 mM, 34% inhibition Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
indole
-
Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ stimulation Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000
-
gel filtration Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
epicotyl
-
Zea mays
-
leaf middle leaves Zea mays
-
shoot
-
Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0441
-
disappearance of 1 nmol indole in 1 min Zea mays

Storage Stability

Storage Stability Organism
4°C, stable for 2 weeks Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
indole + O2 4 atoms of oxygen utilized in disappearance of 1 mol of indole, 2 atoms of oxygen required for initial cleavage of indole and one each for the oxidation of o-aminobenzaldehyde to anthranil and anthranilic acid, simultaneously Zea mays anthranilic acid
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Zea mays

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 6 stable Zea mays

Cofactor

Cofactor Comment Organism Structure
FAD slight stimulation Zea mays