General Stability | Organism |
---|---|
pH 7, 0.02 M sodium phosphate buffer, 0.1 mM EDTA, 6 h in the cold, slight loss of activity | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 0.5 mM, 80% inhibition, Cu2+ protects | Zea mays | |
Cys | 0.5 mM, 78% inhibition, Cu2+ protects | Zea mays | |
diethyldithiocarbamate | reversed by Cu2+ | Zea mays | |
Dithionite | 1 mM, almost complete inhibition | Zea mays | |
Fe2+ | 0.5 mM, slight inhibition | Zea mays | |
Fe3+ | 0.5 mM, slight inhibition | Zea mays | |
GSH | 0.5 mM, 81% inhibition, Cu2+ protects | Zea mays | |
Hg2+ | 0.5 mM, slight inhibition | Zea mays | |
NEM | 0.5 mM, 34% inhibition | Zea mays | |
Salicylaldoxime | 0.5 mM, 34% inhibition | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
indole | - |
Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | stimulation | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98000 | - |
gel filtration | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epicotyl | - |
Zea mays | - |
leaf | middle leaves | Zea mays | - |
shoot | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0441 | - |
disappearance of 1 nmol indole in 1 min | Zea mays |
Storage Stability | Organism |
---|---|
4°C, stable for 2 weeks | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
indole + O2 | 4 atoms of oxygen utilized in disappearance of 1 mol of indole, 2 atoms of oxygen required for initial cleavage of indole and one each for the oxidation of o-aminobenzaldehyde to anthranil and anthranilic acid, simultaneously | Zea mays | anthranilic acid | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Zea mays |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | stable | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | slight stimulation | Zea mays |