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Literature summary for 1.13.11.16 extracted from

  • Spence, E.L.; Kawamukai, M.; Sanvoisin, J.; Braven, H.; Bugg, T.D.
    Catechol dioxygenases from Escherichia coli (MhpB) and Alcaligenes eutrophus (MpcI): sequence analysis and biochemical properties of a third family of extradiol dioxygenases (1996), J. Bacteriol., 178, 5249-5256.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.16

General Stability

General Stability Organism
activity stable in crude extract, inactive apoenzyme stable, can be re-activated by treatment with Fe2+ and ascorbate, irreversible apoenzyme activity loss if stored in completely Fe2+-free buffer, re-activated holoenzyme unstable Cupriavidus necator
activity stable in crude extract, inactive apoenzyme stable, can be re-activated by treatment with Fe2+ and ascorbate, irreversible apoenzyme activity loss if stored in completely Fe2+-free buffer, re-activated holoenzyme unstable Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
diethyl dicarbonate
-
 Cupriavidus necator
diethyl dicarbonate
-
 Escherichia coli
DTNB
-
 Cupriavidus necator
DTNB
-
 Escherichia coli
p-hydroxymercuribenzoate
-
 Cupriavidus necator
p-hydroxymercuribenzoate
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0071
-
 3-(2,3-dihydroxyphenyl)propionate
-
 Cupriavidus necator
0.014
-
 2,3-dihydroxycinnamic acid
-
 Cupriavidus necator
0.036
-
 2,3-dihydroxycinnamic acid
-
 Escherichia coli
0.037
-
 methyl-2,3-dihydroxyphenylpropionate
-
 Escherichia coli
0.059
-
 3-ethylcatechol
-
 Cupriavidus necator
0.094
-
 3-phenethylcatechol
-
 Escherichia coli
0.13
-
 3-methylcatechol
-
 Cupriavidus necator
0.154
-
 3-propylcatechol
-
 Escherichia coli
0.185
-
 3-ethylcatechol
-
 Escherichia coli
0.3
-
 2,3-dihydroxyphenoxyacetic acid
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-(2,3-dihydroxyphenyl)propionate + O2 Cupriavidus necator
-
 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate
-
 ?
3-(2,3-dihydroxyphenyl)propionate + O2 Escherichia coli
-
 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate
-
 ?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-
Escherichia coli P0ABR9 gene mhpB
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cupriavidus necator

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3
-
 2,3-dihydroxyphenylproponic acid Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxycinnamic acid + O2
-
 Cupriavidus necator 2-hydroxy-6-oxonona-2,4,7-trienoate
-
 ?
2,3-dihydroxycinnamic acid + O2
-
 Escherichia coli 2-hydroxy-6-oxonona-2,4,7-trienoate
-
 ?
2,3-dihydroxyphenoxyacetic acid + O2
-
 Escherichia coli 2-hydroxyhexa-2,4-dienoic acid-6-carboxymethylester
-
 ?
2,3-dihydroxyphenylpropionic acid + O2
-
 Cupriavidus necator 2-hydroxy-6-oxonona-dienoate
-
 ?
2,3-dihydroxyphenylpropionic acid + O2
-
 Escherichia coli 2-hydroxy-6-oxonona-dienoate
-
 ?
3-(2,3-dihydroxyphenyl)propionate + O2
-
 Cupriavidus necator 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate
-
 ?
3-(2,3-dihydroxyphenyl)propionate + O2
-
 Escherichia coli 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate
-
 ?
3-ethylcatechol + O2
-
 Cupriavidus necator 2-hydroxy-6-oxoocta-2,4-dienoate
-
 ?
3-ethylcatechol + O2
-
 Escherichia coli 2-hydroxy-6-oxoocta-2,4-dienoate
-
 ?
3-methylcatechol + O2
-
 Cupriavidus necator 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
3-methylcatechol + O2 meta-ring cleavage Escherichia coli 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
3-phenethylcatechol + O2
-
 Escherichia coli 2-hydroxy-6-oxo-8-phenylocta-2,4-dienoate
-
 ?
3-propylcatechol + O2
-
 Escherichia coli 2-hydroxy-6-oxonona-2,4-dienoate
-
 ?
methyl-(2,3-dihydroxyphenyl)propionate + O2
-
 Escherichia coli (2Z,4E)-2-hydroxy-9-methoxy-6,9-dioxonona-2,4-dienoic acid
-
 ?