Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes expressed in Escherichia coli | Brevibacterium fuscum |
Protein Variants | Comment | Organism |
---|---|---|
H200A | Km-value for 3,4-dihydroxyphenylacetate is 6fold lower than wild-type value, kcat for 3,4-dihydroxyphenylacetate is 33.3fold lower than wild-type value | Brevibacterium fuscum |
H200E | Km-value for 3,4-dihydroxyphenylacetate is 75fold lower than wild-type value, kcat for 3,4-dihydroxyphenylacetate is 11.1fold lower than wild-type value | Brevibacterium fuscum |
H200F | Km-value for 3,4-dihydroxyphenylacetate is similar to wild-type value, kcat for 3,4-dihydroxyphenylacetate is 47.6fold lower than wild-type value | Brevibacterium fuscum |
H200N | Km-value for 3,4-dihydroxyphenylacetate is 3fold lower than wild-type value, kcat for 3,4-dihydroxyphenylacetate is 3.33fold lower than wild-type value | Brevibacterium fuscum |
H200Q | Km-value for 3,4-dihydroxyphenylacetate is similar to wild-type value, kcat for 3,4-dihydroxyphenylacetate is 2.5fold lower than wild-type value | Brevibacterium fuscum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
3,4-Dihydroxyphenylacetate | 24°C, pH 7.5, mutant enzyme H200E | Brevibacterium fuscum | |
0.005 | - |
3,4-Dihydroxyphenylacetate | 24°C, pH 7.5, mutant enzyme H200A | Brevibacterium fuscum | |
0.01 | - |
3,4-Dihydroxyphenylacetate | 24°C, pH 7.5, mutant enzyme H20N | Brevibacterium fuscum | |
0.028 | - |
3,4-Dihydroxyphenylacetate | 24°C, pH 7.5, mutant enzyme H200Q | Brevibacterium fuscum | |
0.03 | - |
3,4-Dihydroxyphenylacetate | 24°C, pH 7.5, wild-type enzyme | Brevibacterium fuscum | |
0.031 | - |
3,4-Dihydroxyphenylacetate | 24°C, pH 7.5, mutant enzyme H200F | Brevibacterium fuscum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | contains an active site Fe(II) | Brevibacterium fuscum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium fuscum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyphenylacetate + O2 | proximal extradiol cleavage of the aromatic ring of the substrate | Brevibacterium fuscum | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2,3-HPCD | - |
Brevibacterium fuscum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
3,4-Dihydroxyphenylacetate | 24°C, pH 7.5, mutant enzyme H200F | Brevibacterium fuscum | |
0.3 | - |
3,4-Dihydroxyphenylacetate | 24°C, pH 7.5, mutant enzyme H200A | Brevibacterium fuscum | |
0.9 | - |
3,4-Dihydroxyphenylacetate | 24°C, pH 7.5, mutant enzyme H200E | Brevibacterium fuscum | |
3 | - |
3,4-Dihydroxyphenylacetate | 24°C, pH 7.5, mutant enzyme H20N | Brevibacterium fuscum | |
4 | - |
3,4-Dihydroxyphenylacetate | 24°C, pH 7.5, mutant enzyme H200Q | Brevibacterium fuscum | |
10 | - |
3,4-Dihydroxyphenylacetate | 24°C, pH 7.5, wild-type enzyme | Brevibacterium fuscum |