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Literature summary for 1.13.11.12 extracted from

  • Maynard, D.; Chibani, K.; Schmidtpott, S.; Seidel, T.; Spross, J.; Viehhauser, A.; Dietz, K.J.
    Biochemical characterization of 13-lipoxygenases of Arabidopsis thaliana (2021), Int. J. Mol. Sci., 22, 10237 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli NiCo21(DE3) cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ complete inhibition at 1 mM; complete inhibition at 1 mM; complete inhibition at 1 mM Arabidopsis thaliana
cis-(+)-12-oxophytodienoic acid isoform LOX2 exhibits 64% residual activity at 2.8 mM; isoform LOX3 exhibits 60% residual activity at 2.8 mM; isoform LOX4 exhibits 21% residual activity at 2.8 mM; isoform LOX6 exhibits 50% residual activity at 2.8 mM Arabidopsis thaliana
Cu2+ complete inhibition at 1 mM; complete inhibition at 1 mM; complete inhibition at 1 mM; isoform LOX2 exhibits 50% residual activity at 1 mM Arabidopsis thaliana
methyl jasmonate isoform LOX2 exhibits 87% residual activity at 2.8 mM; isoform LOX3 exhibits 85% residual activity at 2.8 mM; isoform LOX4 exhibits 95% residual activity at 2.8 mM; isoform LOX6 exhibits 94% residual activity at 2.8 mM Arabidopsis thaliana
additional information isoform LOX3 is not inhibited by traumatic acid and salicylic acid; isoform LOX4 is not inhibited by traumatic acid and salicylic acid; isoform LOX4 is not inhibited by traumatic acid and salicylic acid Arabidopsis thaliana
N-ethylmaleimide isoform LOX2 exhibits 84% residual activity at 2.8 mM; isoform LOX3 exhibits 51% residual activity at 2.8 mM; isoform LOX4 exhibits 11% residual activity at 2.8 mM; isoform LOX6 exhibits 47% residual activity at 2.8 mM Arabidopsis thaliana
salicylic acid isoform LOX2 exhibits 96% residual activity at 2.8 mM Arabidopsis thaliana
traumatic acid isoform LOX2 exhibits 95% residual activity at 2.8 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 alpha-linolenic acid isoform LOX2, at pH 7.2 and 25°C Arabidopsis thaliana
0.0012
-
 alpha-linolenic acid isoform LOX6, at pH 7.2 and 25°C Arabidopsis thaliana
0.0058
-
 alpha-linolenic acid isoform LOX2, at pH 7.2 and 25°C Arabidopsis thaliana
0.0058
-
 alpha-linolenic acid isoform LOX4, at pH 7.2 and 25°C Arabidopsis thaliana
0.007
-
 alpha-linolenic acid isoform LOX2, at pH 7.2 and 25°C Arabidopsis thaliana
0.007
-
 alpha-linolenic acid isoform LOX3, at pH 7.2 and 25°C Arabidopsis thaliana
0.0263
-
 alpha-linolenic acid isoform LOX2, at pH 7.2 and 25°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ isoform LOX2 exhibits 690% activity at 1 mM Arabidopsis thaliana
Ca2+ isoform LOX3 exhibits 117% activity at 1 mM Arabidopsis thaliana
Ca2+ isoform LOX6 exhibits 121% activity at 1 mM Arabidopsis thaliana
Cd2+ isoform LOX2 exhibits 220% activity at 1 mM Arabidopsis thaliana
Iron contains non-heme iron Arabidopsis thaliana
Mg2+ isoform LOX2 exhibits 170% activity at 1 mM Arabidopsis thaliana
Mg2+ isoform LOX3 exhibits 135% activity at 1 mM Arabidopsis thaliana
Mg2+ isoform LOX4 exhibits 106% activity at 1 mM Arabidopsis thaliana
Mg2+ isoform LOX6 exhibits 142% activity at 1 mM Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-linolenic acid + O2 Arabidopsis thaliana
-
 13-S-hydroperoxyoctadeca-9, 11,15-trienoic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P38418
-
-
Arabidopsis thaliana Q9CAG3
-
-
Arabidopsis thaliana Q9FNX8
-
-
Arabidopsis thaliana Q9LNR3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-linolenic acid + O2
-
 Arabidopsis thaliana 13-S-hydroperoxyoctadeca-9, 11,15-trienoic acid
-
 ?
alpha-linolenic acid + O2 100% activity Arabidopsis thaliana 13-S-hydroperoxyoctadeca-9, 11,15-trienoic acid
-
 ?
arachidonic acid + O2 isoform LOX3 shows 5% activity compared to alpha-linolenic acid Arabidopsis thaliana ?
-
 ?
arachidonic acid + O2 isoform LOX4 shows 4% activity compared to alpha-linolenic acid Arabidopsis thaliana ?
-
 ?
arachidonic acid + O2 isoform LOX6 shows 8% activity compared to alpha-linolenic acid Arabidopsis thaliana ?
-
 ?
arachidonic acid + O2 isoform LOX2 shows 50% activity compared to alpha-linolenic acid Arabidopsis thaliana 15-(S)-hydroperoxy-5(Z),8(Z),11(Z),13(E)-eicosatetraenoic acid
-
 ?
docosahexaenoic acid + O2 isoform LOX2 shows 56% activity compared to alpha-linolenic acid Arabidopsis thaliana ?
-
 ?
docosahexaenoic acid + O2 isoform LOX3 shows 7% activity compared to alpha-linolenic acid Arabidopsis thaliana ?
-
 ?
docosahexaenoic acid + O2 isoform LOX4 shows 8% activity compared to alpha-linolenic acid Arabidopsis thaliana ?
-
 ?
docosahexaenoic acid + O2 isoform LOX6 shows 31% activity compared to alpha-linolenic acid Arabidopsis thaliana ?
-
 ?
linoleic acid + O2 isoform LOX2 shows 79% activity compared to alpha-linolenic acid Arabidopsis thaliana ?
-
 ?
linoleic acid + O2 isoform LOX3 shows 12% activity compared to alpha-linolenic acid Arabidopsis thaliana ?
-
 ?
linoleic acid + O2 isoform LOX4 shows 11% activity compared to alpha-linolenic acid Arabidopsis thaliana ?
-
 ?
linoleic acid + O2 isoform LOX6 shows 17% activity compared to alpha-linolenic acid Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
? x * 100912, isoform LOX6, calculated from amino acid sequence Arabidopsis thaliana
? x * 95061, isoform LOX2, calculated from amino acid sequence Arabidopsis thaliana
? x * 96791, isoform LOX3, calculated from amino acid sequence Arabidopsis thaliana
? x * 97830, isoform LOX4, calculated from amino acid sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
13-lipoxygenase
-
 Arabidopsis thaliana
13-LOX
-
 Arabidopsis thaliana
LOX2 isoform Arabidopsis thaliana
LOX3 isoform Arabidopsis thaliana
LOX4 isoform Arabidopsis thaliana
LOX6 isoform Arabidopsis thaliana

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.048
-
 isoform LOX4, at pH 7.2 and 25°C Arabidopsis thaliana cis-(+)-12-oxophytodienoic acid
1
-
 isoform LOX2, at pH 7.2 and 25°C Arabidopsis thaliana Cu2+
2.8
-
 isoform LOX6, at pH 7.2 and 25°C Arabidopsis thaliana cis-(+)-12-oxophytodienoic acid