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Literature summary for 1.13.11.12 extracted from
- Characterization of an omega-6 linoleate lipoxygenase from Burkholderia thailandensis and its application in the production of 13-hydroxyoctadecadienoic acid (2015), Appl. Microbiol. Biotechnol., 99, 5487-5497 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| methanol | the enzyme activity is the highest when methanol is used as solvent, optimal at 6% | Burkholderia thailandensis |  |
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | application of the enzyme in the production of 13-hydroxyoctadecadienoic acid | Burkholderia thailandensis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain ER2566 | Burkholderia thailandensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | inhibits at 1 mM | Burkholderia thailandensis | |
| Cu2+ | required for optimal activity, activates at 0.1 mM, inhibits at 1 mM | Burkholderia thailandensis | |
| Fe2+ | inhibits at 1 mM | Burkholderia thailandensis | |
| Ni2+ | inhibits at 1 mM | Burkholderia thailandensis | |
| Zn2+ | activates at 1 mM, inhibits at 0.1 mM | Burkholderia thailandensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Burkholderia thailandensis | |
| 0.0415 | - |
linoleate | pH 7.5, 25°C | Burkholderia thailandensis | |
| 0.0598 | - |
alpha-linolenate | pH 7.5, 25°C | Burkholderia thailandensis | |
| 0.0881 | - |
gamma-linolenate | pH 7.5, 25°C | Burkholderia thailandensis | |
| 0.0978 | - |
arachidonate | pH 7.5, 25°C | Burkholderia thailandensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates at 1 mM | Burkholderia thailandensis | |
| Cu2+ | required for optimal activity, activates at 0.1 mM, inhibits at 1 mM | Burkholderia thailandensis | |
| Fe2+ | required, a non-heme-iron-containing enzyme, inhibits at 1 mM of exogenous Fe2+ | Burkholderia thailandensis | |
| Mg2+ | activates at 1 mM | Burkholderia thailandensis | |
| Zn2+ | activates at 1 mM, inhibits at 0.1 mM | Burkholderia thailandensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
gel filtration | Burkholderia thailandensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-linolenate + O2 | Burkholderia thailandensis | - |
(9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate | - |
? | |
| alpha-linolenate + O2 | Burkholderia thailandensis KCTC 23190 | - |
(9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate | - |
? | |
| arachidonate + O2 | Burkholderia thailandensis | - |
? | - |
? | |
| arachidonate + O2 | Burkholderia thailandensis KCTC 23190 | - |
? | - |
? | |
| gamma-linolenate + O2 | Burkholderia thailandensis | - |
? | - |
? | |
| linoleate + O2 | Burkholderia thailandensis | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | - |
? | |
| linoleate + O2 | Burkholderia thailandensis KCTC 23190 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia thailandensis | - |
- |
- |
| Burkholderia thailandensis KCTC 23190 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain ER2566 by HisTrap affinity chromatographyand dialysis | Burkholderia thailandensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.9 | - |
purified His-tagged enzyme, pH 7.5, 25°C, with substrate gamma-linolenic acid | Burkholderia thailandensis |
| 10.5 | - |
purified His-tagged enzyme, pH 7.5, 25°C, with substrate alpha-linolenic acid | Burkholderia thailandensis |
| 22.6 | - |
purified His-tagged enzyme, pH 7.5, 25°C, with substrate arachidonic acid | Burkholderia thailandensis |
| 26.4 | - |
purified His-tagged enzyme, pH 7.5, 25°C, with substrate linoleic acid | Burkholderia thailandensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-linolenate + O2 | - |
Burkholderia thailandensis | (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate | - |
? | |
| alpha-linolenate + O2 | - |
Burkholderia thailandensis KCTC 23190 | (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate | - |
? | |
| arachidonate + O2 | - |
Burkholderia thailandensis | ? | - |
? | |
| arachidonate + O2 | - |
Burkholderia thailandensis KCTC 23190 | ? | - |
? | |
| gamma-linolenate + O2 | - |
Burkholderia thailandensis | ? | - |
? | |
| linoleate + O2 | - |
Burkholderia thailandensis | (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | - |
? | |
| linoleate + O2 | - |
Burkholderia thailandensis KCTC 23190 | (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | - |
? | |
| additional information | substrate specificity, overview | Burkholderia thailandensis | ? | - |
? | |
| additional information | the optimal substrate concentration in the enzyme assay is 20 g/l | Burkholderia thailandensis | ? | - |
? | |
| additional information | substrate specificity, overview | Burkholderia thailandensis KCTC 23190 | ? | - |
? | |
| additional information | the optimal substrate concentration in the enzyme assay is 20 g/l | Burkholderia thailandensis KCTC 23190 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 75000, SDS-PAGE | Burkholderia thailandensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| linoleate 13S-lipoxygenase | - |
Burkholderia thailandensis |
| omega-6 linoleate lipoxygenase | - |
Burkholderia thailandensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Burkholderia thailandensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 40 | over 50% of maximal activity within this range | Burkholderia thailandensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | thermal inactivation of the purified recombinant enzyme follows first-order kinetics, with half-lives for linoleic acid of 900, 122, 51, and 21 min at 20°C, 25°C, 30°C, 35!C, and 40°C, respectively. Instability of the enzyme above 30°C | Burkholderia thailandensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 13.6 | - |
gamma-linolenate | pH 7.5, 25°C | Burkholderia thailandensis | |
| 42 | - |
alpha-linolenate | pH 7.5, 25°C | Burkholderia thailandensis | |
| 93.7 | - |
linoleate | pH 7.5, 25°C | Burkholderia thailandensis | |
| 112 | - |
arachidonate | pH 7.5, 25°C | Burkholderia thailandensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Burkholderia thailandensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | over 50% of maximal activity at pH 6.5-8.0, 40% of maximal activity at pH 8.5 | Burkholderia thailandensis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | enzyme homology modeling using the crystal structure of linoleate 13-lipoxygenase from Pseudomonas aeruginosa, PDB entry 4G32, as a template | Burkholderia thailandensis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 150 | - |
gamma-linolenate | pH 7.5, 25°C | Burkholderia thailandensis | |
| 700 | - |
alpha-linolenate | pH 7.5, 25°C | Burkholderia thailandensis | |
| 1150 | - |
arachidonate | pH 7.5, 25°C | Burkholderia thailandensis | |
| 2260 | - |
linoleate | pH 7.5, 25°C | Burkholderia thailandensis | |
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