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Literature summary for 1.13.11.12 extracted from

  • Brodhun, F.; Cristobal-Sarramian, A.; Zabel, S.; Newie, J.; Hamberg, M.; Feussner, I.
    An iron 13S-lipoxygenase with an alpha-linolenic acid specific hydroperoxidase activity from Fusarium oxysporum (2013), PLoS ONE, 8, e64919.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.12

Cloned(Commentary)

Cloned (Comment) Organism
gene FoxLOX, DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence comparison, recombinnat expression of His-tagged enzyme Fusarium oxysporum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Fusarium oxysporum
0.00337
-
 (5Z,8Z,11Z,14Z)-eicosatetra-5,8,11,14-enoic acid pH 8.0, 22°C Fusarium oxysporum
0.00689
-
 (9Z,12Z)-octadeca-9,12-dienoic acid pH 8.0, 22°C Fusarium oxysporum
0.0316
-
 (9Z,12Z,15Z)-9,12,15-octadecatrienoic acid pH 8.0, 22°C Fusarium oxysporum
0.0348
-
 (6Z,9Z,12Z)-octadeca-6,9,12-trienoic acid pH 8.0, 22°C Fusarium oxysporum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ weakly active site-bound, required for catalysis, non-heme iron lipoxygenase Fusarium oxysporum
additional information specifically binds iron but not manganese Fusarium oxysporum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
8500
-
 2 * 8500, recombinant His-tagged enzyme, SDS-PAGE Fusarium oxysporum
160000
-
 about, recombinant His-tagged enzyme, gel filtration Fusarium oxysporum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
linoleate + O2 Fusarium oxysporum
-
 (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate
-
 ?
linoleate + O2 Fusarium oxysporum 4287
-
 (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate
-
 ?
additional information Fusarium oxysporum product specificity of FoxLOX, by SP-HPLC/DAD analysis, overview ?
-
 ?
additional information Fusarium oxysporum 4287 product specificity of FoxLOX, by SP-HPLC/DAD analysis, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum
-
 f. sp. lycopersici
-
Fusarium oxysporum 4287
-
 f. sp. lycopersici
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Fusarium oxysporum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,8Z,11Z,14Z)-eicosatetra-5,8,11,14-enoic acid + O2
-
 Fusarium oxysporum ?
-
 ?
(5Z,8Z,11Z,14Z)-eicosatetra-5,8,11,14-enoic acid + O2
-
 Fusarium oxysporum 4287 ?
-
 ?
(6Z,9Z,12Z)-octadeca-6,9,12-trienoic acid + O2
-
 Fusarium oxysporum ?
-
 ?
(9Z,12Z)-octadeca-9,12-dienoic acid + O2
-
 Fusarium oxysporum ?
-
 ?
(9Z,12Z)-octadeca-9,12-dienoic acid + O2
-
 Fusarium oxysporum 4287 ?
-
 ?
(9Z,12Z,15Z)-9,12,15-octadecatrienoic acid + O2
-
 Fusarium oxysporum (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate
-
 ?
(9Z,12Z,15Z)-9,12,15-octadecatrienoic acid + O2
-
 Fusarium oxysporum 4287 (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate
-
 ?
linoleate + O2
-
 Fusarium oxysporum (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate
-
 ?
linoleate + O2
-
 Fusarium oxysporum 4287 (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate
-
 ?
additional information product specificity of FoxLOX, by SP-HPLC/DAD analysis, overview Fusarium oxysporum ?
-
 ?
additional information product specificity of FoxLOX, by SP-HPLC/DAD analysis, overview Fusarium oxysporum 4287 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 8500, recombinant His-tagged enzyme, SDS-PAGE Fusarium oxysporum

Synonyms

Synonyms Comment Organism
13-lipoxygenase
-
 Fusarium oxysporum
iron 13S-lipoxygenase
-
 Fusarium oxysporum
LOX
-
 Fusarium oxysporum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperaature Fusarium oxysporum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.566
-
 (9Z,12Z,15Z)-9,12,15-octadecatrienoic acid pH 8.0, 22°C Fusarium oxysporum
1.71
-
 (5Z,8Z,11Z,14Z)-eicosatetra-5,8,11,14-enoic acid pH 8.0, 22°C Fusarium oxysporum
2.611
-
 (9Z,12Z)-octadeca-9,12-dienoic acid pH 8.0, 22°C Fusarium oxysporum
18.58
-
 (6Z,9Z,12Z)-octadeca-6,9,12-trienoic acid pH 8.0, 22°C Fusarium oxysporum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Fusarium oxysporum

pH Range

pH Minimum pH Maximum Comment Organism
7 10.5 activity range, overview. Low activity below pH 7.0, maximum activity at pH 8.0, 75% of maximal activity at up to pH 10.5 Fusarium oxysporum

General Information

General Information Comment Organism
additional information non-heme iron lipoxygenase oxidizes C18-polyunsaturated fatty acids to 13S-hydroperoxy derivatives by an antarafacial reaction mechanism where the bis-allylic hydrogen abstraction is the rate-limiting step Fusarium oxysporum