Literature summary for 1.13.11.12 extracted from

Jacquot, C.; McGinley, C.M.; Plata, E.; Holman, T.R.; van der Donk, W.A.
Synthesis of 11-thialinoleic acid and 14-thialinoleic acid, inhibitors of soybean and human lipoxygenases (2008), Org. Biomol. Chem., 6, 4242-4252.

Search for more literature for 1.13.11.12

Cloned(Commentary)

Cloned (Comment)	Organism
Escherichia coli BL21 cells carrying the plasmid PT7-7/L1 VTSLO-11	Glycine max

Inhibitors

Inhibitors	Comment	Organism	Structure
11-thialinoleic acid	is a competitive inhibitor with respect to linoleic acid and a noncompetitive inhibitor with respect to arachidonic acid	Glycine max
14-thialinoleic acid	is a competitive inhibitor with respect to linoleic acid	Glycine max
additional information	presence of inhibitor does not change the regioselectivity of lipoxygenase-1	Glycine max

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.028	-	linoleic acid	-	Glycine max

Organism

Organism	UniProt	Comment	Textmining
Glycine max	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by sonication, centrifugation and gel filtration	Glycine max

Storage Stability

Storage Stability	Organism
-80°C, 100 mM borate buffer, pH 10, 30% glycerol	Glycine max

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
linoleic acid + O2	-	Glycine max	(9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoic acid	-	?

Synonyms

Synonyms	Comment	Organism
lipoxygenase-1	-	Glycine max
LO-1	-	Glycine max

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
10	-	-	Glycine max

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.022	-	11-thialinoleic acid	-	Glycine max
0.035	-	14-thialinoleic acid	-	Glycine max

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Release 2023.1 (January 2023)