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Literature summary for 1.13.11.1 extracted from

  • Rodriguez-Salazar, J.; Almeida-Juarez, A.G.; Ornelas-Ocampo, K.; Millan-Lopez, S.; Raga-Carbajal, E.; Rodriguez-Mejia, J.L.; Muriel-Millan, L.F.; Godoy-Lozano, E.E.; Rivera-Gomez, N.; Rudino-Pinera, E.; Pardo-Lopez, L.
    Characterization of a novel functional trimeric catechol 1,2-dioxygenase from a Pseudomonas stutzeri isolated from the gulf of Mexico (2020), Front. Microbiol., 11, 1100 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Stutzerimonas stutzeri

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1.3 mM, 30 min, 39% loss of activity Stutzerimonas stutzeri
Co2+ 1.3 mM, 30 min, 24% loss of activity Stutzerimonas stutzeri
Cu2+ 1.3 mM, 30 min, 42% loss of activity Stutzerimonas stutzeri
EDTA 1.3 mM, 30 min, 29% loss of activity Stutzerimonas stutzeri
Fe2+ 1.3 mM, 30 min, 20% loss of activity Stutzerimonas stutzeri
Fe3+ 1.3 mM, 30 min, 96% loss of activity Stutzerimonas stutzeri
Hg2+ 1.3 mM, 30 min, complete loss of activity Stutzerimonas stutzeri
K+ 1.3 mM, 30 min, 28% loss of activity Stutzerimonas stutzeri
Mg2+ 1.3 mM, 30 min, 40% loss of activity Stutzerimonas stutzeri
Mn2+ 1.3 mM, 30 min, 51% loss of activity Stutzerimonas stutzeri
NaCl increasing NaCl concentration gradually reduce activity. In 0.7 M NaCl quaternary structur changes to a dimeric arrangement and causes a 51% decrease in specific activity on catechol substrate Stutzerimonas stutzeri
Ni2+ 1.3 mM, 30 min, 28% loss of activity Stutzerimonas stutzeri
Zn2+ 1.3 mM, 30 min, 92% loss of activity Stutzerimonas stutzeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0132
-
 catechol pH 8.5, temperature not specified in the publication Stutzerimonas stutzeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
107000
-
 gel filtation Stutzerimonas stutzeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
catechol + O2 Stutzerimonas stutzeri
-
 cis,cis-muconate
-
 ?
catechol + O2 Stutzerimonas stutzeri GOM2
-
 cis,cis-muconate
-
 ?

Organism

Organism UniProt Comment Textmining
Stutzerimonas stutzeri A0A6A9R3Z2 i.e. Pseudomonas stutzeri
-
Stutzerimonas stutzeri GOM2 A0A6A9R3Z2 i.e. Pseudomonas stutzeri
-

Purification (Commentary)

Purification (Comment) Organism
-
 Stutzerimonas stutzeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15
-
 pH 8.5, temperature not specified in the publication Stutzerimonas stutzeri

Storage Stability

Storage Stability Organism
4°C, 6 weeks, 39% loss of activity Stutzerimonas stutzeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
catechol + O2
-
 Stutzerimonas stutzeri cis,cis-muconate
-
 ?
catechol + O2
-
 Stutzerimonas stutzeri GOM2 cis,cis-muconate
-
 ?

Subunits

Subunits Comment Organism
dimer in 0.7 M NaCl quaternary structure changes to a dimeric arrangement and causes a 51% decrease in specific activity on catechol substrate Stutzerimonas stutzeri
trimer 3 * 35000, SDS-PAGE Stutzerimonas stutzeri

Synonyms

Synonyms Comment Organism
C12DO
-
 Stutzerimonas stutzeri
CatA
-
 Stutzerimonas stutzeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Stutzerimonas stutzeri

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45 20°C: about 40% of maximal activity, 45°C: about 25% of maximal activity Stutzerimonas stutzeri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 45 1 h, no loss of activity Stutzerimonas stutzeri
50
-
 30 min, 50% loss of activity. 80% loss of activity after 1 h Stutzerimonas stutzeri
60
-
 10 min, 90% loss of activity. Complete inactivation after 30 min Stutzerimonas stutzeri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16.13
-
 catechol pH 8.5, temperature not specified in the publication Stutzerimonas stutzeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Stutzerimonas stutzeri

pH Range

pH Minimum pH Maximum Comment Organism
7 11.5 pH 7.0: about 70% of maximal activity, pH 11.5: about 75% of maximal activity Stutzerimonas stutzeri