Cloned (Comment) | Organism |
---|---|
gene catA, DNA and amino acid sequence determination and analysis, phylogenetic tree | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | Pseudomonas putida | - |
cis,cis-muconate | - |
? | |
catechol + O2 | Pseudomonas putida N6 | - |
cis,cis-muconate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | A7LBQ4 | strain isolated from the activated sludge samples of a sewage treatment plant at Bentwood Furniture Factory Jasienica, Poland, gene catA | - |
Pseudomonas putida N6 | A7LBQ4 | strain isolated from the activated sludge samples of a sewage treatment plant at Bentwood Furniture Factory Jasienica, Poland, gene catA | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | when cells of strain N6 grow on protocatechuate or 4-hydroxybenzoic acid mainly protocatechuate 3,4-dioxygenases induced. The activity of catechol 1,2-dioxygenase is rather small. The cells grown on benzoic acid, catechol or phenol show high activity of only catechol 1,2-dioxygenase | Pseudomonas putida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
672 | - |
purified enzyme, pH 7.4, 35°C | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoic acid + O2 | - |
Pseudomonas putida | ? | - |
? | |
3,4-dihydroxybenzoic acid + O2 | - |
Pseudomonas putida N6 | ? | - |
? | |
4-hydroxybenzoic acid + O2 | - |
Pseudomonas putida | ? | - |
? | |
4-hydroxybenzoic acid + O2 | - |
Pseudomonas putida N6 | ? | - |
? | |
benzoic acid + O2 | - |
Pseudomonas putida | ? | - |
? | |
catechol + O2 | - |
Pseudomonas putida | cis,cis-muconate | - |
? | |
catechol + O2 | - |
Pseudomonas putida N6 | cis,cis-muconate | - |
? | |
additional information | no activity with vanillic acid | Pseudomonas putida | ? | - |
? | |
additional information | no activity with vanillic acid | Pseudomonas putida N6 | ? | - |
? | |
phenol + O2 | best substrate | Pseudomonas putida | ? | - |
? | |
phenol + O2 | best substrate | Pseudomonas putida N6 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structure prediction, overview | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Pseudomonas putida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
6 | 60 | activity range, profile overview | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | activity range, profile overview | Pseudomonas putida |