Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.1 extracted from

  • Matsumura, E.; Ooi, S.; Murakami, S.; Takenaka, S.; Aoki, K.
    Constitutive synthesis, purification, and characterization of catechol 1,2-dioxygenase from the aniline-assimilating bacterium Rhodococcus sp. AN-22 (2004), J. Biosci. Bioeng., 98, 71-76.
    View publication on PubMed
Search for more literature for 1.13.11.1

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 0.1 mM, enzyme from aniline-grown cells loses 88% of its activity, enzyme from L-malate-grown cells loses 67% of its activity Rhodococcus sp.
HgCl2 enzyme from aniline-grown cells completely loses activity, enzyme from L-malate-grown cells loses 98% of its activity Rhodococcus sp.
PCMB 1.0 mM, enzyme from aniline-grown cells loses 20% of its activity, enzyme from L-malate-grown cells loses 10% of its activity Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 1 * 32000, enzyme from aniline-grown cells and from L-malate-grown cells, SDS-PAGE Rhodococcus sp.
33000
-
 enzyme from aniline-grown cells and from L-malate-grown cells, gel filtration Rhodococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rhodococcus erythropolis inducible on aniline, not on L-malate ?
-
 ?
additional information Frateuria sp. inducible on aniline, not on L-malate ?
-
 ?
additional information Arthrobacter sp. inducible on benzamide, not on L-malate ?
-
 ?
additional information Rhodococcus sp. synthesized constitutively. When the bacterium is incubated on a medium with D-glucose, L-malate, isoleucine, leucine, etc., it synthesizes more catechol 1,2-dioxygenase than that in cells grown on aniline ?
-
 ?
additional information Frateuria sp. ANA-18 inducible on aniline, not on L-malate ?
-
 ?
additional information Arthrobacter sp. BA-5-17 inducible on benzamide, not on L-malate ?
-
 ?
additional information Rhodococcus erythropolis AN-13 inducible on aniline, not on L-malate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
 BA-5-17
-
Arthrobacter sp. BA-5-17
-
 BA-5-17
-
Frateuria sp.
-
 ANA-18
-
Frateuria sp. ANA-18
-
 ANA-18
-
Rhodococcus erythropolis
-
 AN-13
-
Rhodococcus erythropolis AN-13
-
 AN-13
-
Rhodococcus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from aniline-grown cells and from L-malate-grown cells Rhodococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
34
-
 enzyme purified from L-malate grown cells Rhodococcus sp.
35
-
 enzyme purified from aniline-grown cells Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2 enzyme from aniline-grown cells: 74% of the activity with catechol, enzyme from L-malate-grown cells: 71% of the activity with catechol Rhodococcus sp. 2-methyl-cis,cis-muconate
-
 ?
4-methylcatechol + O2 enzyme from aniline-grown cells: 86% of the activity with catechol, enzyme from L-malate-grown cells: 87% of the activity with catechol Rhodococcus sp. 3-methyl-cis,cis-muconate
-
 ?
catechol + O2
-
 Arthrobacter sp. cis,cis-muconate
-
 ?
catechol + O2
-
 Rhodococcus sp. cis,cis-muconate
-
 ?
catechol + O2
-
 Rhodococcus erythropolis cis,cis-muconate
-
 ?
catechol + O2
-
 Frateuria sp. cis,cis-muconate
-
 ?
catechol + O2
-
 Frateuria sp. ANA-18 cis,cis-muconate
-
 ?
catechol + O2
-
 Arthrobacter sp. BA-5-17 cis,cis-muconate
-
 ?
catechol + O2
-
 Rhodococcus erythropolis AN-13 cis,cis-muconate
-
 ?
additional information inducible on aniline, not on L-malate Rhodococcus erythropolis ?
-
 ?
additional information inducible on aniline, not on L-malate Frateuria sp. ?
-
 ?
additional information inducible on benzamide, not on L-malate Arthrobacter sp. ?
-
 ?
additional information synthesized constitutively. When the bacterium is incubated on a medium with D-glucose, L-malate, isoleucine, leucine, etc., it synthesizes more catechol 1,2-dioxygenase than that in cells grown on aniline Rhodococcus sp. ?
-
 ?
additional information inducible on aniline, not on L-malate Frateuria sp. ANA-18 ?
-
 ?
additional information inducible on benzamide, not on L-malate Arthrobacter sp. BA-5-17 ?
-
 ?
additional information inducible on aniline, not on L-malate Rhodococcus erythropolis AN-13 ?
-
 ?
pyrogallol + O2
-
 Rhodococcus sp. alpha-hydroxymuconic acid
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 32000, enzyme from aniline-grown cells and from L-malate-grown cells, SDS-PAGE Rhodococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 enzyme maintains more than 80% of its activity Rhodococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 enzyme from L-malate grown cells Rhodococcus sp.
8.5
-
 enzyme from aniline-grown cells Rhodococcus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 11.6 enzyme maintains more than 90% of its activity Rhodococcus sp.