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Literature summary for 1.13.11.1 extracted from

  • Dorn, E.; Knackmuss, H.J.
    Chemical structure and biodegradability of halogenated aromatic compounds. Two catechol 1,2-dioxygenases from a 3-chlorobenzoate-grown pseudomonad (1978), Biochem. J., 174, 73-84.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.1

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ pyrocatechase I: in absence of 2-mercaptoethanol, pyrocatechase II: with 2-mercaptoethanol, 20 hours incubation time Pseudomonas sp.
Hg2+
-
 Pseudomonas sp.
p-chloromercuribenzoate pyrocatechase I: in absence of 2-mercaptoethanol, pyrocatechase II: with 2-mercaptoethanol, 20 hours incubation time Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 pyrocatechase I, density-gradient centrifugation Pseudomonas sp.
82000
-
 pyrocatechase II, density-gradient centrifugation Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. B 13
-
-
-

Purification (Commentary)

Purification (Comment) Organism
pyrocatechase I and II respectively Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.121
-
 3-chlorocatechol Pseudomonas sp.
0.172
-
 4-chlorocatechol Pseudomonas sp.
0.348
-
 pyrocatechase II, at room temperature, instable to chromatography and dilution Pseudomonas sp.
0.64
-
 catechol Pseudomonas sp.
20
-
 pyrocatechase I, catechol Pseudomonas sp.

Storage Stability

Storage Stability Organism
pyrocatechase I: 4°C, Tris-HCl buffer, pH 8.0, supplemented with 2-mercaptoethanol, stable over a period of 3 months Pseudomonas sp.
pyrocatechase II: at room temperature, Tris-HCl buffer, pH 8.0, supplemented with 2-mercaptoethanol, 10% loss of activity after 3 weeks, at -16°C for 24 hours 95% loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chlorocatechol + O2 pyrocatechase II, in 3-chlorobenzoate-grown cells Pseudomonas sp. 2-chloro-cis,cis-muconate
-
 ?
3-chlorocatechol + O2 pyrocatechase II, in 3-chlorobenzoate-grown cells Pseudomonas sp. B 13 2-chloro-cis,cis-muconate
-
 ?
4-chlorocatechol + O2 pyrocatechase II, in 3-chlorobenzoate-grown cells Pseudomonas sp. (2E,4Z)-3-chlorohexa-2,4-dienedioate
-
 ?
4-chlorocatechol + O2 pyrocatechase II, in 3-chlorobenzoate-grown cells Pseudomonas sp. B 13 (2E,4Z)-3-chlorohexa-2,4-dienedioate
-
 ?
catechol + O2
-
 Pseudomonas sp. cis,cis-muconate
-
 ?
catechol + O2
-
 Pseudomonas sp. B 13 cis,cis-muconate
-
 ?
additional information extradiol cleavage in addition to intradiol fission at a ratio 1:14 with pyrocatechase I, not with pyrocatechase II Pseudomonas sp. ?
-
 ?
additional information extradiol cleavage in addition to intradiol fission at a ratio 1:14 with pyrocatechase I, not with pyrocatechase II Pseudomonas sp. B 13 ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Pseudomonas sp.