Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Hydrogenimonas thermophila | 16020 | - |
periplasm | localized in both, the periplasmic space and membrane | Hydrogenimonas thermophila | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE | Hydrogenimonas thermophila |
86300 | - |
gel filtration | Hydrogenimonas thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hydrogenimonas thermophila | - |
- |
- |
Hydrogenimonas thermophila EPI-55-1%T | - |
- |
- |
Oxidation Stability | Organism |
---|---|
the purified enzyme retains 37% of its initial activity after 72 h of air exposure. After 96 h of air exposure, no activity of the purified enzyme is detected | Hydrogenimonas thermophila |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, Biogel hydroxyapatite column chromatography, and Superdex 200 gel filtration | Hydrogenimonas thermophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
crude extract, at 55°C, pH 7.0, using methylene blue as electron acceptor | Hydrogenimonas thermophila |
30 | - |
after 152fold purification, at 55°C, pH 7.0, using dichloroindophenol as electron acceptor | Hydrogenimonas thermophila |
55 | - |
after 152fold purification, at 55°C, pH 7.0, using methyl viologen as electron acceptor | Hydrogenimonas thermophila |
344 | - |
after 152fold purification, at 55°C, pH 7.0, using methylene blue as electron acceptor | Hydrogenimonas thermophila |
484 | - |
after 152fold purification, at 80°C, pH 7.0, using methylene blue as electron acceptor | Hydrogenimonas thermophila |
1078 | - |
after 152fold purification, at 55°C, pH 7.0, using phenazine methosulfate as electron acceptor | Hydrogenimonas thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + methyl viologen | - |
Hydrogenimonas thermophila | H+ + reduced methyl viologen | - |
? | |
H2 + methyl viologen | - |
Hydrogenimonas thermophila EPI-55-1%T | H+ + reduced methyl viologen | - |
? | |
H2 + oxidized dichloroindophenol | - |
Hydrogenimonas thermophila | H+ + reduced dichloroindophenol | - |
? | |
H2 + oxidized dichloroindophenol | - |
Hydrogenimonas thermophila EPI-55-1%T | H+ + reduced dichloroindophenol | - |
? | |
H2 + oxidized methylene blue | - |
Hydrogenimonas thermophila | H+ + reduced methylene blue | - |
? | |
H2 + oxidized methylene blue | - |
Hydrogenimonas thermophila EPI-55-1%T | H+ + reduced methylene blue | - |
? | |
H2 + oxidized sodium hydrosulfite | - |
Hydrogenimonas thermophila | H+ + reduced sodium hydrosulfite | - |
? | |
H2 + oxidized sodium hydrosulfite | - |
Hydrogenimonas thermophila EPI-55-1%T | H+ + reduced sodium hydrosulfite | - |
? | |
H2 + phenazine methosulfate | best electron acceptor | Hydrogenimonas thermophila | H+ + reduced phenazine methosulfate | - |
? | |
additional information | no activity is observed using NAD+, 2,3-dimethoxy methyl-1,4-benzoquinone, and potassium ferricyanide as electron acceptors | Hydrogenimonas thermophila | ? | - |
? | |
additional information | no activity is observed using NAD+, 2,3-dimethoxy methyl-1,4-benzoquinone, and potassium ferricyanide as electron acceptors | Hydrogenimonas thermophila EPI-55-1%T | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | - |
Hydrogenimonas thermophila |
Synonyms | Comment | Organism |
---|---|---|
[NiFe] hydrogenase | - |
Hydrogenimonas thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
at pH 6.0, using 10 mM methyl viologen or 20 mM sodium hydrosulfite as electron acceptors | Hydrogenimonas thermophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 90 | at 90°C the enzyme retains 83% of maximum activity, at lower temperatures hydrogenase activity remains the same as at 10°C (13% of maximum activity), similar activity is observed between 40 and 60°C (about 70% of maximum activity) | Hydrogenimonas thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
50 mM Tris-HCl | Hydrogenimonas thermophila |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10.5 | at pH 9.5 activity decreases to 28% of activity at pH 9.0, hydrogenase activity decreases completely at pH 10.5, while at pH 6.0 45% of activity is retained | Hydrogenimonas thermophila |