Literature summary for 1.12.99.6 extracted from

Lenz, O.; Zebger, I.; Hamann, J.; Hildebrandt, P.; Friedrich, B.
Carbamoylphosphate serves as the source of CN(-), but not of the intrinsic CO in the active site of the regulatory [NiFe]-hydrogenase from Ralstonia eutropha (2007), FEBS Lett., 581, 3322-3326.

Search for more literature for 1.12.99.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli JM109 cells	Cupriavidus necator

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Cupriavidus necator	5737	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	within the catalytic centre one carbonyl and two cyanide ligands are covalently attached to the iron	Cupriavidus necator

Organism

Organism	UniProt	Comment	Textmining
Cupriavidus necator	-	-	-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Strep-Tactin Superflow column chromatography	Cupriavidus necator

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2 + acceptor	-	Cupriavidus necator	H+ + reduced acceptor	-	?
H2 + acceptor	-	Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1	H+ + reduced acceptor	-	?

Synonyms

Synonyms	Comment	Organism
regulatory hydrogenase	-	Cupriavidus necator
[NiFe]-hydrogenase	-	Cupriavidus necator

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Release 2023.1 (January 2023)