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Literature summary for 1.12.99.6 extracted from

  • Dementin, S.; Burlat, B.; De Lacey, A.L.; Pardo, A.; Adryanczyk-Perrier, G.; Guigliarelli, B.; Fernandez, V.M.; Rousset, M.
    A Glutamate is the essential proton transfer gate during the catalytic cycle of the [NiFe] hydrogenase (2004), J. Biol. Chem., 279, 10508-10513.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Solidesulfovibrio fructosivorans

Protein Variants

Protein Variants Comment Organism
E25D approx. 50% of wild-type H2 uptake activity Solidesulfovibrio fructosivorans
E25Q less than 0.1% of wild-type H2 uptake activity Solidesulfovibrio fructosivorans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe contains FeNi-center Solidesulfovibrio fructosivorans
Ni contains FeNi-center Solidesulfovibrio fructosivorans

Organism

Organism UniProt Comment Textmining
Solidesulfovibrio fructosivorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Strep-tag enzyme Solidesulfovibrio fructosivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + oxidized methyl viologen
-
Solidesulfovibrio fructosivorans reduced methyl viologen + H+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Solidesulfovibrio fructosivorans

pH Range

pH Minimum pH Maximum Comment Organism
6 11 almost no activity at pH 6.0, approx. 30% of maximal activity at pH 11.0 Solidesulfovibrio fructosivorans