Literature summary for 1.12.99.6 extracted from

Chardin, B.; Giudici-Orticoni, M.T.; De Luca, G.; Guigliarelli, B.; Bruschi, M.
Hydrogenases in sulfate-reducing bacteria function as chromate reductase (2003), Appl. Microbiol. Biotechnol., 63, 315-321.

Search for more literature for 1.12.99.6

Inhibitors

Inhibitors	Comment	Organism	Structure
Cr3+	weak inhibition	Desulfomicrobium norvegicum
Cr3+	weak inhibition	Desulfovibrio vulgaris
Cr6+	0.03 mM, complete inhibition of methyl viologen reduction	Desulfomicrobium norvegicum
Cr6+	0.1 mM, complete inhibition of methyl viologen reduction	Desulfovibrio vulgaris

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.016	-	Cr6+	pH 7.0	Desulfovibrio vulgaris
0.016	-	Cr6+	pH 7.0	Solidesulfovibrio fructosivorans
0.021	-	Cr6+	pH 7.0	Desulfomicrobium norvegicum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe	contains several [4Fe-4S] clusters	Desulfovibrio vulgaris
Fe	contains several [4Fe-4S] clusters	Desulfomicrobium norvegicum
Fe	contains several [4Fe-4S] clusters	Solidesulfovibrio fructosivorans
Ni	contains Ni in the catalytic center	Desulfomicrobium norvegicum
Ni	contains Ni in the catalytic center	Solidesulfovibrio fructosivorans

Organism

Organism	UniProt	Comment	Textmining
Desulfomicrobium norvegicum	-	-	-
Desulfovibrio vulgaris	-	-	-
Solidesulfovibrio fructosivorans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Desulfovibrio vulgaris
-	Desulfomicrobium norvegicum
-	Solidesulfovibrio fructosivorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
80	-	H2 uptake	Solidesulfovibrio fructosivorans
80	-	Cr6+ reduction	Solidesulfovibrio fructosivorans
161	-	Cr6+ reduction	Desulfomicrobium norvegicum
205	-	H2 uptake	Desulfomicrobium norvegicum
7680	-	Cr6+ reduction	Desulfovibrio vulgaris
50000	-	H2 uptake	Desulfovibrio vulgaris

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2 + Cr6+	-	Desulfovibrio vulgaris	Cr3+ + H+	-	?
H2 + Cr6+	-	Desulfomicrobium norvegicum	Cr3+ + H+	-	?
H2 + Cr6+	-	Solidesulfovibrio fructosivorans	Cr3+ + H+	-	?
H2 + oxidized methyl viologen	-	Desulfovibrio vulgaris	reduced methyl viologen + H+	-	?
H2 + oxidized methyl viologen	-	Desulfomicrobium norvegicum	reduced methyl viologen + H+	-	?
H2 + oxidized methyl viologen	-	Solidesulfovibrio fructosivorans	reduced methyl viologen + H+	-	?

Synonyms

Synonyms	Comment	Organism
Fe-hydrogenase	-	Desulfovibrio vulgaris
NiFe-hydrogenase	-	Solidesulfovibrio fructosivorans
NIFeSe-hydrogenase	-	Desulfomicrobium norvegicum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.09	-	Cr6+	pH 7.0	Solidesulfovibrio fructosivorans
0.23	-	Cr6+	pH 7.0	Desulfomicrobium norvegicum
1.3	-	Cr6+	pH 7.0	Desulfovibrio vulgaris

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Release 2023.1 (January 2023)