General Stability | Organism |
---|---|
90% loss of activity after 8 days in the presence of oxygen, increasing ionic strength causes 10-40% reversible inhibition | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 95% inhibition at 0.1 mM | Escherichia coli | |
N-bromosuccinimide | complete inactivation at 10 mM, 20% inactivation at 0.1 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
H2 | - |
Escherichia coli | |
0.0045 | - |
benzyl viologen | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | 12.2 mol per mol enzyme, iron-sulfur protein | Escherichia coli | |
Sulfide | 9.1 mol per mol enzyme, iron-sulfur protein | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
1 * 64000, 1 * 31000, 1 * 29000, SDS-PAGE | Escherichia coli |
31000 | - |
1 * 64000, 1 * 31000, 1 * 29000, SDS-PAGE | Escherichia coli |
64000 | - |
1 * 64000, 1 * 31000, 1 * 29000, SDS-PAGE | Escherichia coli |
200000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.6 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + acceptor | benzyl viologen, methylene blue and methylviologen can act as electron acceptors | Escherichia coli | H+ + reduced acceptor | - |
? | |
H2 + oxidized benzyl viologen | - |
Escherichia coli | H+ + reduced benzyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 64000, 1 * 31000, 1 * 29000, SDS-PAGE | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | enzyme is still 60% active after 4 h at pH 4 or pH 10 | Escherichia coli |