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Literature summary for 1.12.99.6 extracted from
- A cytoplasmic nickel-iron hydrogenase with high specific activity from Desulfovibrio multispirans sp. n., a new species of sulfate reducing bacterium (1984), Biochem. Biophys. Res. Commun., 125, 1025-1032.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.41 | - |
H2 | with methyl viologen as electron acceptor | Desulfovibrio multispirans |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Desulfovibrio multispirans | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | 11 atoms per mol enzyme | Desulfovibrio multispirans | |
| Ni | 0.9 atoms per mol enzyme | Desulfovibrio multispirans | |
| Sulfide | 10 atoms labile sulfide per mol enzyme | Desulfovibrio multispirans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24500 | - |
1 * 58000 + 1 * 24500, SDS-PAGE | Desulfovibrio multispirans |
| 58000 | - |
1 * 58000 + 1 * 24500, SDS-PAGE | Desulfovibrio multispirans |
| 82000 | - |
gel filtration | Desulfovibrio multispirans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Desulfovibrio multispirans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| apparent homogeneity | Desulfovibrio multispirans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 792 | - |
- |
Desulfovibrio multispirans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + acceptor | enzyme is more active in hydrogen evolution than in hydrogen uptake | Desulfovibrio multispirans | H+ + reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 58000 + 1 * 24500, SDS-PAGE | Desulfovibrio multispirans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Desulfovibrio multispirans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable for 60 min | Desulfovibrio multispirans |
| 70 | - |
stable for 20 min | Desulfovibrio multispirans |
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Release 2023.1 (January 2023)
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