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Literature summary for 1.12.99.6 extracted from

  • Bernhard, M.; Buhrke, T.; Bleijlevens, B.; De Lacey, A.L.; Fernandez, V.M.; Albracht, S.P.J.; Friedrich, B.
    The H2 sensor of Ralstonia eutropha. Biochemical characteristics, spectroscopic properties, and its interaction with a histidine protein kinase (2001), J. Biol. Chem., 276, 15592-15597.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
25
-
H2
-
Cupriavidus necator

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Cupriavidus necator 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 4Fe-4S cluster Cupriavidus necator
Ni NiFe-hydrogenase Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
alpha2,beta2, 2 * 37000 + 2 * 55000, SDS-PAGE Cupriavidus necator
55000
-
alpha2,beta2, 2 * 37000 + 2 * 55000, SDS-PAGE Cupriavidus necator
165000
-
gel filtration Cupriavidus necator

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-

Oxidation Stability

Oxidation Stability Organism
enzyme is extremely sensitive to inactivation by O2 Cupriavidus necator

Purification (Commentary)

Purification (Comment) Organism
purified protein forms a complex with histidine protein kinase Cupriavidus necator

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.942
-
-
Cupriavidus necator

Storage Stability

Storage Stability Organism
4°C, 20% loss of activity after 48 h under argon Cupriavidus necator
4°C, 50% loss of activity after 12 h under an atmosphere of 100% H2 Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + acceptor benzyl viologen acts as electron acceptor Cupriavidus necator H+ + reduced acceptor
-
?

Subunits

Subunits Comment Organism
tetramer alpha2,beta2, 2 * 37000 + 2 * 55000, SDS-PAGE Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
H2 production Cupriavidus necator

pH Range

pH Minimum pH Maximum Comment Organism
5 10 H2 uptake is constant over a pH range from pH 5 to pH 10 Cupriavidus necator