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Literature summary for 1.12.98.B1 extracted from

  • Roy, R.; Adams, M.W.
    Characterization of a fourth tungsten-containing enzyme from the hyperthermophilic archaeon Pyrococcus furiosus (2002), J. Bacteriol., 184, 6952-6956.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

Metals/Ions Comment Organism Structure
Calcium tungsten-containing enzyme, contains approximately one tungsten three iron, three or four acid-labile sulfide, and one cacium atom per subunit Pyrococcus furiosus
Iron tungsten-containing enzyme, contains approximately one tungsten three iron, three or four acid-labile sulfide, and one cacium atom per subunit. Presence of an unusual iron-sulfur chromophore Pyrococcus furiosus
Tungsten tungsten-containing enzyme, contains approximately one tungsten three iron, three or four acid-labile sulfide, and one cacium atom per subunit Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
2 * 69000, SDS-PAGE Pyrococcus furiosus
69363
-
2 * 69363, calculated from sequence Pyrococcus furiosus
129000
-
gel filtration Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus O93736
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
additional information
-
the specific tungsten content is 14.2 nmol/mg, 119fold purification Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + sulfur
-
Pyrococcus furiosus H2S
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 69000, SDS-PAGE Pyrococcus furiosus
homodimer 2 * 69363, calculated from sequence Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
PF1961
-
Pyrococcus furiosus
WOR 4
-
Pyrococcus furiosus

General Information

General Information Comment Organism
physiological function the enzyme has a role in sulfur metabolism Pyrococcus furiosus